"The facility provides a wide variety of options for the analysis and quantification of fluorescent probes, markers and antibodies within fixed and living cells."
Lab would benefit from lab tracking/ software system. I suggested LabLife.com; Lab contact person will be changing as of 6/1

Morehouse School of Medicine
720 Westview Dr. SW
Atlanta, GA 30310

Morehouse School of Medicine
MEB 332
720 Westview Dr. SW
Atlanta, GA 30310

TEJ: moved all of lab delivery address to one cell; added "Facility" to lab name; added dashes to contact phone; do we have a new lab contact person?

TEJ: added label, per conversation with RNav

TEJ: created row for this instrument per term requested for the 633 laser.

TEJ: added label and changed type ("Lasers HeNe 633" is not part of ontology); term request refers to label for separate instrument.

TEJ: added type -- added term request as synonym in ontology. EJS: added company to label.

TEJ: term request filled. EJS: added company to label.

TEJ: type used (LED light source) is not part of ontology -- annotated to "light source". EJS: added company to label.

Millipore Cytofluor Multiwell Fluorescence Plate Reader

The Biomedical Technology Service Laboratory (BTSL) Core facility has been in existence for more than twenty years. The BTSL operates in multiple locations which house basic, multi-use equipment, available to all researchers, including: autoclaves, centrifuges, incubators for bacteria and cell cultures, spectrophotometers, scintillation counters, plate readers, luminometer, sonicators and microscopes. The BTSL also provides liquid nitrogen, dry and wet ice, glassware washers and laundry facilities.

waiting for lab director to email updated equipment inventory list Carlo: wrong format of first and Last name

"The Biomedical Technology Service Laboratory (BTSL) / Multi-Use Instrumentation Facility is a core facility that provides multiple research resources, and equipment support for the biomedical and clinical research faculty at Morehouse School of Medicine."

MEB C-241
720 Westview Drive SW
Atlanta, GA 30310

TEJ: updated URL; added lab documentation file name
Biomedical Technology Service Core Laboratory

"Major support for the biomedical research infrastructure of Morehouse School of Medicine is received through the Research Centers for Minority Institutions (RCMI) Program, sponsored by the National Centers for Research Resources of the National Institutes of Health."

720 Westview Drive SW
Atlanta, GA 30310-1495

Research Centers in Minority Institutions (RCMI), Morehouse School Of Medicine

Has expertise in: "1D & 2D Gel Electrophoresis, Capillary Electrophoresis, Multi-Use Equipment maintenance and instruction."

"The Allegra 6 Series is designed for high-volume labs requiring a simple, durable solution above all else. Save time with the basic, streamlined user interface that is easy to use and quick to program. Available in refrigerated and ambient benchtop and refrigerated knee-well models."

TEJ: Removed freezers. These are not invisible resources and do not need to be cataloged.
AM: Updated name, description, technique, model, website

Are these rotors or separate centrifuges?

Are these rotors or separate centrifuges?
AM: Updated name, technique

Add the instrument type Centrifuge. EJS: added model number.
AM: Updated name, technique

Beckman Coulter Microfuge R microcentrifuge

Carlo: Deleted term request, moved the value into labe and add instrument type TEJ: annotated to newly added "CO2 Incubator" term

"The Molecular Devices SpectraMax 250 has an optical design that is the only multi-channel system that truly mimics a dual-beam spectrophotometer. Each sample has a discrete sample beam and reference beam so that each well is measured directly. The eight-channel system- consisting of 8 sample beams and 8 reference beams delivers both superior precision and speed of reading across the microplate. Up to 96 assays can be run at one time and users can perform spectral scans from 250 nm to 750 nm."
Carlo: Fixed instrument type changed label into the term request value TEJ: added manufacturer data
Molecular Devices, Inc. SpectraMax 250 Microplate reader

Carlo: changed the label (used the instrument type). EJS: added System to end of label.
Barnstead NANOpure water purification system

TEJ: updated type. EJS: added manufacturer.

TEJ: Term request filled; ammended manufacturer data

Two copies of this instrument available.

changed the label, fixed the document type

changed label, fixed instrument type, removed term request

"The patented multichannel optical design of the SpectraMax® 190 Absorbance Microplate Reader mimics a dual beam spectrophotometer, measures each sample in the plate directly, and eliminates measurement error due to variations in light output between optical fibers on other instruments for more reliable data reporting. The eight-channel system delivers both high precision and speed when reading 96-well microplates for endpoint measurements, kinetic assays, and spectral scans.

The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including DNA quantitation in the UV range."

TEJ: added instrument per attached word doc
SpectraMax 190 Absorbance microplate reader

"The Agilent 8453 UV-Visible spectrophotometer uses a photodiode array (PDA) for simultaneous measurement of the complete ultra-violet to visible light spectrum in less than one second. The PDA technique brings exceptional reliability and repeatability. Using Agilent ChemStation software you get powerful data analysis and data archival to ensure compliance with good laboratory practices (GLP) and pharmacopoeia requirements."

TEJ: added instrument per attached word doc
AM: Updated name, type, description, technique, website

TEJ: added instrument per attached word doc. EJS: added model number.

TEJ: added instrument per attached word doc. EJS: added company to label.
AM: Updated name, technique

Beckman Coulter LS 6500 liquid scintillation counter

TEJ: added instrument per attached word doc. EJS: added manufacturer.

Perkin Elmer Lambda 3B UV/VIS Spectrophotometer

Includes cell sorting and bio-image analysis.
TEJ: formatted name; swapped protocols and labels

TEJ: swapped protocols and labels. NV: Filled term request

Examples of polyclonal/monoclonal antibodies produced in this lab have been antibodies against conjugated synthetic peptides, viral proteins, chemokines, cytokines, cellular antigens, and protein bands excised from polyacrylamide gels.
Permission from individual researchers is needed to share their antibodies.
Monoclonal/polyclonal antibody production

TEJ: swapped protocols and labels

MH: changed type to material processing service. added techniques.

TEJ: swapped protocols and labels
MH: changed type to material storage service.

(404) 752-1146 -- old phone number still valid?

Service includes: analysis and quantification of fluorescent probes, markers and antibodies within fixed and living cells.

"Access to CLAR facilities is restricted to authorized investigators with CLAR and IACUC approval. No individuals may enter the facilities without this CLAR/IACUC authorization.

Since its inception, the Center for Laboratory Animal Resources (CLAR) has centralized most research and teaching animal care for the Atlanta University Center consortium. Our purpose is to provide total animal care services as needed to all academic and/or research faculty and staff affiliated with any one of the member institutions of the Atlanta University Center (AUC)."

Hugh Gloster Building
720 Westview Dr. SW
Atlanta, GA 30310

Mr Hazel is Laboratory Animal Health Technician in the Center for Laboratory Animal Resources at MSM.

Center for Laboratory Animal Resources
Morehouse School of Medicine
720 Westview Dr. SW
Atlanta GA 30310-1495

EJS: updated annotation with filled term request.

Includes feeding, watering, and cleaning of primary and secondary animal enclosures.
Available to all academic and/or research faculty and staff affiliated with any one of the member institutions of the Atlanta University Center (AUC).

NV: Moved data from geographic restriction to restriction.
MH: changed type.

NV: Moved data from geographic restriction to restriction.

Includes disposal of all animal species upon termination of experimental protocol.

NV: Moved data from geographic restriction to restriction.
MH changed type, title added technique.

Includes maintenance of immunologically compromised animals behind specified barriers, i.e. Specific Pathogen Free (S.P.F.) rabbits, thymectomized mice etc.

NV: Moved data from geographic restriction to restriction.
MH changed type and label.

Service provided by CLAR within the per diem charges.

NV: Moved data from geographic restriction to restriction.
MH changed type and technique.

Routine vaccination against common pathogens, i.e. Sendai, I.C.H. etc., will be absorbed by CLAR.

NV: Moved data from geographic restriction to restriction.

MH: changed type.

Includes consultation on veterinary, regulatory, and/or animal model aspects of laboratory animal use, as requested by investigators.

Service includes assistance with breeding.

NV: Moved data from geographic restriction to restriction.
MH changed type.

NV: Moved data from geographic restriction to restriction.
MH changed type, added technique, changed label.

NV: Moved data from geographic restriction to restriction.
MH. changed type. added technique.

NV: Moved data from geographic restriction to restriction. Added technique.

Ms. Donna Floyd is Laboratory Animal Health Technician in the Center for Laboratory Animal Resources at MSM.

Dr. Emmett is expert in the analysis of Gene Expression using microarray-based technology, and is scientific consultant to the Functional Genomics (microarray) lab, in the Gene Profiling core at MSM.

Ms. Leander-Griffith is Research Assistant in the Functional Genomics (Microarray) lab, Gene Profiling Core at MSM.

Room C-241, Medical Education Building
Morehouse School of Medicine
720 Westview Dr. SW
Atlanta Ga 30310-1495

"The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells on a single platform. Results are delivered within 30-40 minutes in automated, high quality digital data."

added type
AM: Updated description, technique, model, website. NV: changed type, added technique.

Robbins Scientific hybridization chamber

The Biomedical Informatics Unit (BIU) of the Morehouse School of Medicine (MSM) RCMI Center of Excellence for Clinical and Translational Research (R- CENTER) focuses on integration of MSM and affiliated health information systems -- electronic medical and personal health records, patient databases, and other research databases (basic, translational, clinical and population-based) -- into an interoperable repository. The long-term goal is to establish an integrated standards-based informatics platform to support MSM clinical and translational research investigators in their own work and in sharing research information with collaborators and other investigators in parallel fields. Ultimately, the interoperable repository platform will expand scientific discovery and research translation of MSM investigators in collaboration with A-CTSI partners as well as the RCMI consortium. The building blocks of the repository have begun with the i2b2 platform.

i2b2 (Informatics for Integrating Biology and the Bedside) is an NIH-funded National Center for Biomedical Computing based at Partners HealthCare System. The i2b2 is a scalable informatics framework that will bridge clinical research data and the vast data banks arising from basic science research in order to better understand the genetic bases of complex diseases. This knowledge will facilitate the design of targeted therapies for individual patients with diseases having genetic origins. i2b2 is being implemented across a number of collaborative research networks. These linked data will serve as a major institutional resource for advancing clinical/translational research, and enhancing career development for clinical/translational research, community health, health services/outcomes and clinical trials. The creation of an integrated data repository will greatly enhance the training and career development goals of the R-CENTER by facilitating ready access to critical research data that will support trainees in the CRECD program, junior investigators, pilot grant awardees and other researchers across the MSM R-CENTER.

The Bioinformatics Lab facilitates analysis of gene expression data gathered from multiplatform microarrays (Agilent, Affymetrix, etc) as well as provides analysis and consultation related to various expression profiling technologies which include RNA, miRNA, ChIP-on-Chip, and Antibody microarrays and post-hoc analysis of SNP and SAGE data.

The primary focus of the Analytical Chemistry Laboratory is to provide reliable and comprehensive analytical services to basic and clinical researchers. These services include:

1. Custom sample analysis by High-performance liquid chromatography (HPLC).
2. ELISA
3. Electrolytes analyses
4. Sample preparation (derivatization and extraction/purification)
5. Data reports and QC reports
6. Consultation in analytical techniques and methodology

Equipment/Technology

1. High Performance Liquid Chromatography (HPLC): Custom analyses by HPLC can include (but not limited to): amino acids; thiols (total, oxidized, reduced); chiral and racemic compounds such as warfarin and clenbuterol; etc.
i. Agilent 1100 series HPLC equipped with a diode array detector (DAD), variable wavelength detector (VWD), 1046 series fluorescence detector.
ii. Agilent 1100 series HPLC system equipped with 1100 series fluorescence detector.
2. Molecular Devices SpectroMax Plus microtiter plate reader and variable wavelength UV-Visible spectrophotometer to perform Colorimetric and Enzyme-linked Immunosorbent assays.
3. NOVA Electrolytes Analyzer for analyses of Na+, P+, TCa+, Li+, Cl- in biological fluids.

Service Charges

The Analytical Chemistry Laboratory performs custom testing and has a standard fee schedule for services. The fee structure is based on the amount of time required to process the samples (extraction, derivatization, etc.) and the cost of supplies and reagents. For non-NIH sponsored projects (MSM and outside institutions and corporations), labor costs are assessed. A complete listing of currently available tests or cost per requested assay can be emailed or faxed upon request. Additionally, since the Analytical Chemistry Laboratory performs custom testing, if the requested assay is not on the list, please contact the core leader directly for test availability and the most up-to-date pricing.

TEJ: is there an actual mailing address?

Dept. of Pharmacology and Toxicology
720 Westview Dr., S.W.
Atlanta, GA 30310

Building MRC, Room 127
720 Westview Dr., S.W.
Atlanta GA 30310

The Analytical Chemistry and Protein Profiling Core is comprised of three service core labs:

A. Analytical Chemistry Lab

The primary focus of the Analytical Chemistry Laboratory is to provide reliable and comprehensive analytical services to basic and clinical researchers. These services include:

1. Custom sample analysis by High-performance liquid chromatography (HPLC).
2. ELISA
3. Electrolytes analyses
4. Sample preparation (derivatization and extraction/purification)
5. Data reports and QC reports
6. Consultation in analytical techniques and methodology

B. Luminex Lab

The Luminex Lab has a Bio-Plex 200 multiplex plate reader, which allows simultaneous analytical measurement of multiple protein biomarkers linked to beads, and provides for exquisite sensitivity, specificity, and precise measurements from small sample volumes.

Services include:
1. Multiplex Immunoassays
2. QuantiGene Multiplex Assays

C. Proteomics Lab

Proteins can be identified by trypsin digestion and subsequent analysis of peptides by LC/MS/MS. Samples can be bands or spots from 1D or 2D gel electrophoresis or solutions of moderate complexity. Expression analysis can be done using ProteoIQ software to compare samples. Pathway analysis can be done in order to identify biological processes and relationships. Analysis of protein translational modifications PTMs can also be done.

not sure how to treat this and other "umbrella cores" that link to labs but don't have their own resources. need to discuss - TJ
Analytical Chemistry & Protein Profiling Core

Lab contains two copies of this instrument, one equipped with a 1100 series Fluorescence Detector (FD) and an Agilent 1049A Electrochemical Detector (ECD), and the other equipped with a Diode Array Detector (DAD), a Variable Wavelength Detector (VWD) and a 1046A Fluorescence Detector (FD).
EJS: added Agilent Technologies to label.

"The patented multichannel optical design of the SpectraMax® Plus384 Absorbance Microplate Reader mimics a dual beam spectrophotometer, measuring each sample in the plate directly, eliminating measurement error due to variations in light output between optical fibers on other instruments for more reliable data reporting. The nine-channel system delivers both high precision and speed when reading cuvettes, 96-well, and 384-well microplates for endpoint measurements, kinetic assays, and spectral scans."
SpectraMax Plus384 Absorbance microplate reader
TEJ: not sure if this should be classified as a microplate reader or a spectrophotometer -- ask the lab? Ok. EJS: added microplate reader to label.

With Flame Ionization Detector (FID) and Nitrogen Phosphorus Detector (NPD).

EJS: added company to label.
AM: Updated name, technique

Agilent 5972 High Sensitivity Mass Spectrometer (MSD)

Allows analysis of Na, K, total Ca,Li, Cl.

HPLC method for determination of ADMA, SDMA and arginine in plasma samples protocol

HPLC method for determination amino acids protocol

HPLC method for determination of p-Aminohippuric (PAH) Acid in plasma and urine samples protocol

Thiols include:
* Homocysteine
* Cysteine
* Glutathione

HPLC assay for vanillin and vanillic acid protocol

Chiral HPLC analysis of R- and S-Warfarin in plasma samples protocol

"SoftMax® Pro Data Acquisition & Analysis Software serves as the user interface to the popular EMax®, VMax™, Gemini™, and SpectraMax® Microplate Readers. Users are able to customize reader settings through the software to take advantage of unique reader technologies that improve the quality of assay data such as: temperature-independent pathlength correction for absorbance measurements and automatic gain correction for fluorescence."
see changes, no term request seems necessary now

Service includes analysis for:
sodium, potassium, total calcium and lithium.

PRP: No, comment in regards to the labs speciality

Provides analysis for 8-isoprostane (in urine), adiponectin total, adiponectin multimeric, TNF, hsCRP, 25-hydroxy vitamin D, 1,25-dihydroxy vitamin D, nitric, oxide, and aldosterone.

High performance liquid chromatography service

"The core lab has a BioPlex bead sorter, which allows the analysis of multiplex assays such as ELISAs from a single, small volume sample."

"The Luminex 100/200 System is a flexible analyzer based on the principles of flow cytometry. The system enables you to multiplex (simultaneously measure) up to 100 analytes in a single microplate well, using very small sample volumes. The system delivers fast and cost-effective bioassay results on many assay formats including nucleic acid assays, receptor-ligand assays, immunoassays and enzymatic assays."

BioPlex (Luminex) analysis is performed for the following proteins:
6 Plex: TNF-a, IL-6, Leptin, MCP-1, HGF, Insulin
2 Plex: Resistin, Total PAI1
1 Plex: Active PAI1
1 Plex: Insulin

TEJ: I took this label from the lab menu word doc, but I'm not sure what it refers to. Is this a company? A type of test? Is there a more descriptive label that would be better? Also I don't know which instruments these tests use?

For over thirty years, Dr. James P. Griffin, Jr. has been involved in behavioral health promotion, training, education, and research. He has an earned doctorate in psychology with specialized training in behavior modification, school psychology, and community/organizational psychology.

He is affiliated with the Metro Atlanta Violence Prevention Partnership (MAVPP)

Community Health & Preventive Medicine
Morehouse School of Medicine
1513 East Cleveland Avenue, Building 500
East Point, Georgia 30344

NV: Filled term request // bioqa: changed type - this is a kit, not a nucleic acid reagent

Recognizes_species
Homo sapiens
Mus musculus

This mouse expresses a fusion protein involving the circadian clock gene Period 2, and firefly luciferase. In the presence of the substrate luciferin, cultured tissue from this mouse emits light in proportion to the presence of Per2, dynamically over time. This is used as an index of circadian clock status in the tissue. Homozygous on a C57BL/6 background.
OTTO KURATOR: converted value of property: http://purl.obolibrary.org/obo/ERO_0000578 to embedded instance. The literal value was: Expresses firefly luciferace in all regions of endogenous period 2 expression

Monoclonal anti-Phosphoserine reacts against phosphorylated serine both as free amino acid or when conjugated to carriers such as BSA or KLH.

No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP.

Research Instructor, Cardiovascular Research Institute & Department of Physiology

Recognizes_species
Gallus gallus
Mus musculus

"Being overweight or obese increases the risk of the development of many diseases such as diabetes and cardiovascular diseases. It is known that the proliferation, differentiation and apoptosis of preadipocytes are all related to the hyperplasia of adipose tissue. Mitochodrial biogenesis has been reported to accompany with brown and white adipose cell differentiation. Prohibitins are highly conserved proteins mostly locating in mitochondria and play important roles in the proliferation, differentiation and apoptosis in a lot of different types of cells. One of the interests in Dr. Liu's laboratory is focused on the role of prohibitins during adipogenesis.

The emerging field of regenerative medicine will require a reliable and accessible source of stem cells in addition to biomaterial scaffolds and cytokine growth factors. Adipose-derived stem cells have demonstrated very similar phenotypic and functional characteristics to that of bone marrow-derived mesenchymal stem cells with the ability to differentiate along multiple lineage pathways besides adipocyte. Another interest in Dong Liu's laboratory is to define the role of prohibitins in regenerative capacity of adult stem cells by testing the effect of prohibitins on adipose-derived stem cells differentiation into cardiomyocytes, endothelial cells and smooth muscle cells."

Morehouse School of Medicine
720 Westview Drive
Atlanta, GA 30310

NV: Deleted additional lab- is now displaying broken link.
Deleted MSM organization- now also displaying as a broken link.

This mouse is heterozygous for Dnmt1 mutation. It's cryopreserved at Jackson Lab.
"Mice homozygous for this mutation die at mid-gestation. The embryos are stunted and show a delayed development. DNA from these mice shows a reduction in the level of m5C to about 1/3 that seen in cells from normal wild type siblings."
(http://jaxmice.jax.org/strain/002123.html)

DNA (Cytosine-5-)-Methyltransferase 1 knockout

Training/breeding animals. A protocol transfer form must accompany any animal requests.

Manually entered from original excel form.
NV: Added technique.

Manually entered from original Excel form.

"Nearly all biological functions, in nearly all organisms, are organized in the time domain. The daily appearance and disappearance of environmental light during evolution provided a framework within which animals could consolidate their activity and rest periods. The timing of behavior and other physiological activity is internalized, and is under the control of internal biological clocks called circadian oscillators.

In my lab, using mouse models, we strive to understand how these rhythms are controlled by the master oscillator in the brain, called the SCN. We also try to understand the consequences of disrupting the normal temporal harmony in the organism. For example, shift work is common in our society, and carries with it numerous health consequences such as increased rates of cancer. Our animal models simulate such lifestyles, and our studies are designed to determine exactly what is causing disease in these environments, and how this can be prevented."

"Dr. Hibbert’s primary research focus is nutritional implications of altered metabolism in sickle cell disease (SCD). Dr. Hibbert’s research projects include: protein, energy and hemoglobin metabolism in stable SCD patients; relationship of metabolic alterations, nutritional status and immune response in SCD; potential health benefits of nutritional supplements in SCD patients and transgenic mouse model; genetic modifiers of SCD severity focusing on pathways for energy and protein metabolism and proinflammatory factors, using stored human plasma and DNA; temporal changes of proinflammatory markers in tissues and effect of L-arginine supplement using the SCD transgenic mouse. Dr. Hibbert collaborates with Dr. Stiles, Dr. Gee and Dr. Newman at MSM and with colleagues at Emory University and Medical College of Georgia."

720 Westview Dr. SW
Atlanta, GA 30310-1045

Sickle cell disease (SCD) is the result of a change in the amino acid composition (mutation) of one or both beta hemoglobin genes. These are transgenic mice that have a knockin of all the human globin genes.
OTTO KURATOR: converted value of property: http://purl.obolibrary.org/obo/ERO_0000578 to embedded instance. The literal value was: C57Bl/6

Berkeley knockout model is a complete knockout of murine alpha and beta globins followed by insertion of a transgene containing human alpha, beta, A gamma, G gamma globins.
Related disease aand Biological process studied fields do not allow new term request- "Sicle cell disease"
5 parental strains including: C57Bl/6, 129, FVB/N, DBA/2, and Black Swiss

"Established in 1985, the RCMI Electron Microscopy Core facility provides complete scanning and transmission services, from specimen fixation/dehydration/critical point drying/ embedding/polymerization/ block trimming/sectioning/slide-making/formvar-coating, to examination and documentation of prepared samples utilizing JEOL 820 scanning and JEOL 1200EX transmission electron microscopes."

"Examination and documentation of prepared specimens utilizing Transmission and/or Scanning Electron Microscope."
Limited to Morehouse School of Medicine and neighboring institutions.

"Our lab is interested in the molecular pathogenesis of HIV/AIDS. Our current projects focus on the role that the viral protein Nef plays in AIDS pathogenesis. We have found that the Nef protein is secreted from infected cells in small membrane-bound packages called "exosomes." These exosomes can fuse with non-infected cells and cause a variety of effects, including programmed cell death. Nef exosomes are also present in the plasma of HIV infected individuals. In collaboration with Dr. Vincent Bond at Morehouse School of Medicine, we are exploring the possibility that Nef exosomes could represent a major source of the T cell depletion, a hallmark of HIV/AIDS. We are currently testing this hypothesis in non-human primates and strategies are currently being devised to develop therapeutic vaccines and anti-virals based on this model. Our lab also provides Luminex assays, mass spectrometry and Proteomics services for the school. Those interested in initiating a project with the Proteomics core should contact Dr. Powell."
Associate Professor, Department of Microbiology, Biochemistry & Immunology
Member of broken link goes back to a deleted record for Morehouse School of Medicine. Cannot manually remove from field. Does not need to be replaced, as MSM was created in error.

Proteins can be identified by trypsin digestion and analysis of peptides by LC/MS/MS. Samples can be bands or spots from 1D or 2D gel electrophoresis or solutions of moderate complexity. Expression analysis can be done using ProteoIQ software to compare samples. Pathways analysis can be done to identifity biological processes and relationships. Analysis of protein translational modifications PTMs can also be done.

"Surface enhanced laser desorption ionization (SELDI) can be used in several different kinds of applications which include, but are not limited to:

1. Protein profiling and Biomarker discovery.
2. Protein-Protein interactions.
3. Protein-Nucleic acid interactions.
4. Identification of proteins by tryptic digest.
5. Determination of the protein properties prior to purification."

Use of Luminex instrument for multi-analytic detection. The Bio-Plex and Miliplex Assay services are offered by the Proteomics core.

"Thermo Scientific* Proteome Discoverer software is a flexible, expandable platform for the analysis of qualitative and quantitative proteomics data.
Outstanding sensitivity and the rapid growth of public databases have made mass spectrometry the primary method of identifying proteins in complex biological samples. While database search algorithms such as SEQUEST* and MASCOT* have existed for over a decade to facilitate the identification of proteins from mass spectral data, the field of proteomics continues to expand and with it the need for more advanced and flexible software tools to interpret the data. Proteome Discoverer was designed to address this need for flexibility by offering a wide array of bio-software tools and customizable workflows for every proteomics experiment."

Synthetic peptide, corresponding to amino acids 432-528

Research Wing, Room 222
720 Westview Drive., S.W.
Atlanta, GA 30310-1495

Recognizes_species
Homo sapiens
Oryctolagus cuniculus
Mus musculus

Synthetic Irx4 peptide based on the sequence: PHSGALDRHQDSPVTSL

Living Colors A.v. Monoclonal Antibody (JL-8)

The DNA Sequencing Lab uses the capillary electrophoresis technology developed by Applied Biosystems (ABI) to resolve fluorescently-labeled DNA on the 16-capillary ABI 3130xl Gene Analyzer. The lab offers both DNA sequencing and fragment (microsatellite) analysis to research users. Options include enumeration of polymorphisms (SNPs) in patient samples, a service which complements SNP discovery protocols offered in the Human Genotyping Lab.

Instrumentation: Applied Biosystems (ABI) 3100xl Gene analyzer, ABI 9600 and Veriti thermocyclers,

Software: Applied Biosystems Suite – (Sequence Analysis 5.2, GeneMapper 4.0, SeqScape 2.5)

Service Charges:
DNA Sequencing: $5/sequencing reaction (MSM),
(reaction and electrophoresis) $6/reaction (non-MSM)
Large-user sequencing discount $4/reaction (MSM)
(full 96-well plate) $5/reaction (non-MSM)
DNA Fragment Analysis: $1.50 per sample (MSM)
(electrophoresis only) $2 per sample (non-MSM)

Room B-315C, Hugh Gloster Bldg.
Morehouse School of Medicine
720 Westview Drive, SW
Atlanta, GA 30310-1495

Room C-241, Medical Education Bldg.
Morehouse School of Medicine
720 Westview Drive, SW
Atlanta, GA 30310-1495

Morehouse School of Medicine
720 Westview Drive
Atlanta, GA 30310-1495

Consists of a minimum of two years working with clinical and/or animal protocols. Methods include stable isotopes, protein analysis, energy expenditure and body composition.
Ph.D. in biochemistry, metabolism or nutrition
Post-Doctoral Fellowship in Hemoglobinopathy

"Morehouse School of Medicine’s strong emphasis on strengthening primary care, promoting prevention and early detection, and working to reduce and eliminate health disparities has made the institution a unique academic medical center in the United States – and the world.

The biotechnologies developed by MSM researchers are targeted to prevent, diagnose and treat chronic diseases like HIV/AIDS and other infectious diseases, cancer, cardiovascular disease, metabolic and neurological disorders. Technology transfer will continue to be an important process for creating new and existing research and development efforts as well as a driving force for national and regional economic development."

Office of Research Administration
720 Westview Drive SW
Research Wing, Room 133
Atlanta, Georgia 30310-1495

Office of Technology Transfer, Morehouse School of Medicine

"Sandra Harris-Hooker, Ph.D., graduated from Dillard University in New Orleans, Louisiana with a degree in Biology. She earned her Ph.D. in Developmental Biology from Atlanta University (now Clark-Atlanta University), in Atlanta, Georgia.

Upon completion of her Ph.D., Dr. Harris-Hooker spent three years of postdoctoral training in the Department of Pathology at the University of Washington School of Medicine, in Seattle, Washington. After training in Seattle, she served on the faculty in the Department of Pathology at Boston University School of Medicine before joining the faculty at Morehouse School of Medicine, in Atlanta, Georgia. As a member of the faculty of Morehouse School of Medicine, Dr. Harris-Hooker is currently Vice President/Associate Dean for Sponsored Research Administration, as well as Professor in the Department of Pathology.

While her primary research area is cardiovascular biology, she is also interested in ways to enhance the integration of basic, clinical and population-based research in order to address disparities in health. To further supplement her interest in addressing disparities in health, Dr. Harris-Hooker serves as Deputy Director for the Center for Excellence on Health Disparities. To bridge the gap between basic, clinical and population-based research, she belongs to a number of organizations, including the International Society on Hypertension in Blacks, the American Heart Association, and the New York Academy of Science. She is also a member of the Morehouse School of Medicine Chapter of Alpha Omega Alpha Medical Honor Society.

Dr. Harris-Hooker serves on several national boards and external committees such as the National Space Biomedical Research Institute, the Association for the Accreditation of Human Research Protection Programs, Inc., the International Society of Hypertension in Blacks, and the Board of Advisors to the Jackson State University College of Science Engineering and Technology."

Vice President/Associate Dean, Sponsored Research Administration

Different fees apply for MSM and non-MSM users. See website for details.
Fragment (microsatellite) analysis service

"Expanded options include enumeration of polymorphisms (SNPs) in patient samples, a service which can be used to complement SNP discovery protocols offered in the Human Genotyping Lab."
Enumeration of single nucleotide polymorphisms

Shaker-incubator for growth of bacterial cultures.

AM: Updated name, type, technique, model

Suite includes:
* Sequence Analysis 5.2
* GeneMapper 4.0
* SeqScape 2.5
* Invitrogen Vector NTi10.3 suite
* Sequencer 4.2
* Geneious 4.0.2

Contact Michael Powell of Jane Chu for LC-MS at the Proteomics Facility and Natalia Silvestrov for GC-MS at the Analytical Chemistry Core.
NV: Filled term request. Changed type. Removed techniques, not sure what this instrument is actually used for but according to the website, it says it's based on flow cytometry technology.

KLH-conjugated synthetic peptide containing the sequence DFme2KTD corresponding to dimethyl-lysine at position 79 of histone H3.

Amino acids 258-327 mapping within an internal region of TBX5

IRX5 partial recombinant protein with GST tag

Synthetic peptide corresponding to amino acids in the middle portion of the second E1A binding domain (B pocket) of human and mouse Rb

Synthetic phospho-peptide (KLH-coupled) corresponding to residues around Ser807/811 of human Rb

The Tech Transfer office provides registration for:

* Literary work
* Visual arts work
* Performing arts work
* Sound recording
* Single serial issue
* Motion picture or other audiovisual
work

The Tech Transfer Office reviews invention disclosures and provides the following intellectual property services to inventors at Morehouse School of Medicine:

* determine whether the disclosure represents intellectual property that is a trade secret, or whether it is an invention that would require patent protection
* ascertain whether the invention disclosure can be adequately evaluated by the intellectual property committee or whether to defer evaluation to an external intellectual property management agency
* institute action to protect the disclosed invention as intellectual property of Morehouse School of Medicine
* transfer intellectual property rights of the described disclosure to the research sponsor, if such transfer is required by the research agreement
* waive ownership of the intellectual property rights regarding the disclosed technology in favor of the inventor and release the inventor from further responsibility to the university with respect to the technology that was disclosed
* request additional pertinent information needed for the committee to evaluate the disclosure. In such circumstances, the committee shall deem the disclosure "not adequate" and the calculation of the time frames shall not begin until the requested information is received by the committee"

Intellectual property disclosure service

The Tech Transfer Office can provide licenses for the following commercialization opportunities:

* TUMOR CYTOTOXICITY INDUCED BY MODULATORS OF THE CXCR4 RECEPTOR - "This invention relates to the use of Gp120 and Nef proteins of retroviruses, or peptides of these proteins, as therapeutic agents against carcinomas."

* METHOD FOR IDENTIFYING SALT-SENSITIVE PERSONS - "This invention relates to evaluation of salt sensitivity as it relates to propensity to present or develop hypertension."

* ANTI –CHEMOKINE AND –ASSOCIATED RECEPTOR ANTIBODIES AND USES FOR INHIBITION OF INFLAMMATION - "This invention relates to antibodies or the use of antibodies, against precise chemokines or chemokine receptors, that mediate certain inflammatory diseases, e.g., chronic obstructive pulmonary disease (COPD), inflammatory bowel disease (IBD), interstitial cystitis."

* ANTI –CHEMOKINE AND –ASSOCIATED RECEPTOR ANTIBODIES AND USES FOR INHIBITION OF CANCER GROWTH AND METASTASIS - "This invention relates to antibodies or the use of antibodies, against precise chemokines or chemokine receptors, to provide a means of inhibiting the growth and/or metastasis of tumor cells."

* TRYPANOSOME DERIVED APOPTOTIC FACTORS (TAF) - "This invention relates to the use of anti-TAF monoclonal antibody to inhibit cell death of microvascular endothelial, brain and other cells. It is the purpose of this invention to provide antibodies that neutralize or abrogate TAF activity."

* MODULATING VACCINE AGAINST HIV-1 NEF PROTEIN INDUCED LYMPHOCYTE DEPLETION - "The invention comprises of a peptide prophylactic or therapeutic vaccine that will provide protection against Nef-mediated pathogenesis, which causes T cell depletion seen in full-blown AIDS."

* NEUREGULINS AS REGULATORS OF STEM CELL MIGRATION - "This invention relates to the administration of neuregulin-1 to mobilize endogenous neuronal stem cells to the damaged areas of the brain, following stroke or other neurodegenerative disorders."

* RAD ATTENUATION OF VASCULAR LESION FORMATION AND TREATMENT OF VASCULAR DISEASES - "It is the purpose of this technology to provide a means
for preventing and treating restenosis and atherosclerosis."

* RAD INHIBITION OF CARDIOMYOCYTE HYPERTROPHY &
USES FOR TREATING OF HEART DISEASES - "This invention describes a method to identify and/or to treat cardiac hypertrophy, respectively."

* CAGE DISPENSER FOR MEASURING FOOD INTAKE IN LABORATORY RODENTS - "This invention relates to use of food dispensers for studies requiring careful measurement of intake by animals."

* SPLICE VARIANTS OF BRCA1 - "This invention relates to the identification of i) a nucleic acid sequence encoding human BRCA1a and BRCA1b, ii) a protein encoded by the nucleic acid sequence of BRCA1a and BRCA1b, iii) a vector comprising the nucleic acid sequence of BRCA1a and BRCA1b, iv) a host cell transfected with BRCA1a and BRCA1b and v) an in vitro method of identifying agents capable of modulating the amount or activity of BRCA1a or BRCA1b proteins in cells."

* NOVEL NANOPARTICLES FOR DELIVERY OF INERT AND BIOLOGICALLY ACTIVE AGENTS - "The current technology uses a novel and relatively inexpensive technique to generate polycaprolactone-coated biopolymer-polyethylene glycol nanoparticles, which allows for controlling the size of the therapeutic or diagnostic particle (100 nm to 50 um; + 10% of mean size), > 99% loading efficiency and polymer- or ligand-coating for controlled-, protected-, and targeted-release of its contents."

* HETEROCYCLIC AND DIPHENYL KETONES HAVING CYTOTOXIC PROPERTIES - "This invention relates to the discovery of novel heterocyclic and dialkoxyphenyl
compounds which possess potent cytotoxic activity."

* A HIGH THROUGHPUT METHOD FOR HAPLOTYPE DETERMINATION - "This invention provides an improved, more reliable method of haplotype determination."

* COMPOSITIONS FOR THE PREVENTION AND TREATMENT OF NEUROINJURY AND METHODS OF USE THEREOF - "This invention relates to post-trauma administration of NRG-1 by any known route of administration following TBI to prevent secondary neuronal injury and inflammation."

* DETECTION OF PLASMODIUM FALCIPARUM ANTIGENS IN SALIVA OF MALARIA PATIENTS - "This invention relates to detection of Plasmodium falciparum antigens in saliva of malaria patients."

* METHODS FOR STORAGE AND RETRIEVAL OF FUNCTIONAL MATURE RETINAL CELLS - "The present invention relates generally to the field of cell isolation and storage and, in particular, to the storage and retrieval of functional mature retinal cells."

* DETECTION OF HIV-RELATED PROTEINS IN URINE - "This invention relates to methods for diagnosis and, in particular, to methods for detecting HIV infection, diagnosing HIV or HIV-associated diseases using biomarkers in the urine."

"This facility is equipped to provide basic histological services including: paraffin embedding, paraffin sections, frozen sections, immunohistochemical and H&E staining, and in situ hybridization. The facility consists of a paraffin tissue embedding center, rotary microtomes, cryostat, tissue floatation bath, microwave, tissue processor, and slide warmers."

Morehouse School of Medicine
Histology Core Laboratory
720 Westview Drive, SW
Atlanta, GA 30310

The Cell and Tissue Imaging Facility provides a broad range of services covering many of the major structural methods used to study cells and tissues. The Core consists of four labs: histological and microscopy training for graduate students and tissue processing and collection services for the research faculty at MSM and other institutions. This facility provides a broad range of services covering many of the major structural methods used to study cells and tissues.

Morehouse School of Medicine
720 Westview Drive, SW
Atlanta, GA 30310

Flow Cytometry & Cell Sorting Laboratory

Flow Cytometry & Cell Sorting Core Leader

"Complete preparation of samples (i.e. fixation, dehydration, critical point drying, sputter coating, embedding, polymerization, knife-making, trimming, H/E slide-preparation, sectioning, formvar-coated grid preparation and
subsequent grid-staining)."

Sample preparation for electron microscopy

Electron microscopy negatives are developed and scanned onto CD for the investigator.

Assistant Professor, Cardiovascular Research Institute & Department of Medicine

NV: Removed:

Our long-term goal is to discover and understand the genetic and epigenetic variations that contribute to predisposition and progression of human diseases and to ethnic disparities. Our research team is composed of physician-scientists, molecular biologists, and software engineers, we are using molecular and cellular biology, genomics and epigenomics, epidemiology, bioinformatic and computational biology approaches. Our current goal is post-GWAS functional analysis to identify causal variants and underlying molecular mechanisms.

Our current projects include work in the following main areas:

Molecular biology studies of SNPs and CNVs on p47phox, Notch3, PDE4D and CRP
DNA methylation in atherosclerosis and diabetes
Technology development for molecular haplotyping
Technology development for high-throughput identification of novel splicings, exons, and genes on GWAS-positive regions
Technology development for high-throughput genome-wide identification of regulatory SNPs in cis-interactions
Software development and computational biology
Integration of genetic and epigenetic datasets
Epigenetic inheritance

"The CVRI has access to facilities well-equipped for physiological, biochemical and molecular biologic studies. Investigators are supported by a full array of state-of-the-art equipment and software.

The CVRI includes a faculty of 12 and an overall research team of 33 individuals. The new CVRI recruits have generated a total annual extramural grant revenue of $3.3 million. Follow the link for contact information and biographies."

"The Human Genotyping Lab assists investigators to apply the genomic technologies to population-based research. The strategic plan is to create a link between basic science, clinical investigation and population-based research by integrating genomic sciences into our research program. The central hypothesis is that health disparities are related in part to ethnicity-specific DNA variants in critical genes that influence the susceptibility to common diseases. Morehouse School of Medicine has a longstanding interest on the studies of ethnic disparities and a longstanding strength on community outreach to underserved minority populations. Accordingly, the major objective of the MSM Human Genome Core Laboratory is to enhance the research capacity on these sample cohorts and upgrade our research to the molecular genetic level based on cutting-edge genomic technologies."
on the web page make MSM note it's Dr. Song, not MS. Qing Song ...error on that page

Morehouse School of Medicine
RW 216
720 Westview Drive
Atlanta, GA 30310

Principal investigator for all RCMI cores at MSM.

Morehouse School of Medicine
720 Westview Drive, SW
Atlanta, GA 30310-1495

Bioinformatic and computational analysis related to SNPs and other genetic variations.

"The investigator is responsible for the fixation and blocking of the tissue. For routine fixation it is recommended that the tissue is fixed with a fresh solution of 10% neutral buffered formalin or paraformaldehyde. For best results, it is recommended that the fixatives be purchased in small quantities. (Contact the laboratory for details)

For frozen tissue preparations contact the laboratory."

Service fee for paraffin sections and embedding may have been put into "relevant" website when it should have gone into "service fee URL"

The Morehouse School of Medicine Functional Genomics Core is an RCMI funded core that provides fee-based microarray ‘-OMICS’ services for research investigators using glass slide technology platforms. We are an Agilent Certified Core facility and a member of the Association of Biomolecular Research Core Facilities (ABRF) worldwide. Specifically we provide assistance, or full services, from RNA isolation to scanning hybridized microarrays and raw data extraction. In conjunction with the RCMI Bioinformatics Core, we provide full services in the analyses of ‘-omics’ datasets. We support all microarray products from Agilent as well as any other array company that utilizes uncoated glass slide technologies. We also provide consultative services for custom microarray design.
Laboratory Notebook, CDs and hard drive for long term storage
LAB INVENTORY SYSTEM is for the user only. Data Investigator driven, would require investigator permission for use

MEB 241
720 Westview Dr.
Atlanta, GA 30310

The Agilent SurePrint G3 Exon Microarrays make it easy to transition from standard
expression analysis to exon analysis. Probes are designed to target all exons within genes based on well-annotated and up-to-date databases, ensuring both comprehensive and relevant coverage across the genome. Our high-quality 60-mer probes ensure high sensitivity and accuracy for all Agilent gene expression microarrays, including the new exon arrays. The Low Input Quick Amp WT Labeling Kit (LIQAWT) makes amplification and labeling from a low amount of RNA quick and easy. Having been co-developed with these arrays, LIQA WT is optimized to maintain an accurate representation of RNA species, now across whole transcripts. In addition, GeneSpring 11.5 provides new features for the analysis of splicing events along with gene-level expression, lending confidence in the detection of subtle changes now possible with the exon microarrays system.

MH curated with Paula- need term request for microarray type.

"The Agilent SurePrint G3 Mouse Exon Microarrays come in two options, offering a choice in coverage and throughput. Offering probes targeting individual exons, these arrays make it possible to look beyond the gene to more subtle but relevant expression changes. Agilent Gene Expression Microarrays remain the fast, simple, and affordable choice in gene expression analysis, with new capabilities to drill down into important RNA changes including those at the exon level. Importantly, the gold standard sensitivity provided by Agilent Gene Expression Microarrays has been retained in these new Exon versions, so that even the smallest fold changes are detected."
Paula- can you add a description, a URL, and a manufacturer? I changed the type.

"Agilent’s high-resolution microarray scanner is part of an improved microarray platform that provides greater transcript definition and chromosomal definition, as well as expression measurements that more accurately reflect the range of gene activity in biological systems. Agilent’s system produces accurate relative quantitation of signal levels over a range of more than six orders of magnitude, far exceeding the capabilities of other systems. This enhances data quality across a broad range of applications such as gene expression, miRNA profiling, array CGH, ChIP, and methylation assays."
Agilent DNA Microarray Scanner with SureScan High Resolution Technology

make this website the same for all of this cores services may have omitted on a couple :-(

Chromatin immunoprecipitation (ChIP) is a method used to determine the location of DNA binding sites on the genome for a particular protein of interest. This technique gives a picture of the protein–DNA interactions that occur inside the nucleus of living cells or tissues. The in vivo nature of this method is in contrast to other approaches traditionally employed to answer the same questions.

not sure this technique is valid

The genomic library is a collection of clones containing the genomic DNA sequences of an organism. Typically, these molecules are propagated in bacteria or phage. The library is an important tool used in the process of isolating genes.

"Applied Biosystems 3130xl Genetic Analyzer is the latest generation of 16-capillary electrophoresis instruments for the medium throughput laboratories. The system offers industry-leading performance, plus sophisticated automation capabilities allowing researchers to save time, reduce costs and increase productivity."

Includes reaction and capillary electrophoresis.
Capillary electrophoresis (CE), also known as capillary zone electrophoresis (CZE), can be used to separate ionic species by their charge and frictional forces and hydrodynamic radius. In traditional electrophoresis, electrically charged analytes move in a conductive liquid medium under the influence of an electric field. Introduced in the 1960s, the technique of capillary electrophoresis (CE) was designed to separate species based on their size to charge ratio in the interior of a small capillary filled with an electrolyte.

"Thermo Scientific LTQ XL high performance linear ion trap delivers more structural information than any other ion trap mass spectrometer. LTQ XL is the only mass spectrometer that offers multiple dissociation techniques, PQD, ETD and CID. Pulsed Q Collision Induced Dissociation (PQD) is a proprietary technique that eliminates the low mass cut-off concern inherent with all ion traps. This results in extensive coverage for predicted and unpredicted metabolites, and the ability to perform peptide quantification using iTRAQ* labels. In addition, fast polarity switching for high-sensitivity analysis of unknowns, high resolution isolation (HRI), intelligent Data Dependent* acquisition, and optional high resolution accurate mass functionality are all tools to help the researcher gain confident structural identification routinely."

"Gel excision instrument, includes enclosure, imaging system, fluidics system, robotics, sensors, cutting head, gel tray, microplate track, wash station."

"The Bio-Plex® 200 system is a suspension array system which offers protein and nucleic acid researchers a reliable multiplex assay solution that permits analysis of up to 100 biomolecules in a single sample."

Available as mRNA expression microarrarys.

Available are mRNA expression microarrarys

Available are mRNA expression, Exon, miRNA, Encode chip-on-chip, promotor, CpG Island, DNA Methylation, CNV, CGH, and CGH+SNP microarrarys.

Available are mRNA expression, Exon, miRNA, promotor, CpG Island, DNA Methylation, CNV, CGH, and CGH microarrarys.

GenUs BioSystems standard gene expression service includes all materials, processing, quality control, and reporting - including follow-up analysis for a single price. Specialists in small, difficult sample handling we regularly handle sample sizes down to 10 cells (Total RNA yield ~ 100 picograms) utilizing state of the art single and double amplification protocols.

Available are mRNA expression, Exon, miRNA, promotor, and CGH microarrarys.
I spelled seqwright wrong on the previous, this is correct

"The Xenopus Microarray project is supported by the Rockefeller University; it is part of the Laboratory of Molecular Vertebrate Embryology headed by Professor Ali Brivanlou. Software tools and database development are provided by the Laboratory of Computational Genomics headed by Assistant Professor Terry Gaasterland."
Please test if you are able to go to the xenopus website..

NV: Website is not working, so I removed it:
http://arrays.rockefeller.edu/xenopus/

"The new BD FACSAria™ II cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications.

The heart of this cell sorting revolution is the true fixed-alignment flow cell, gel-coupled to the collection optics. Fixed alignment
minimizes startup time and improves reproducibility. Gel coupling to the objective lens improves collection efficiency and increases the sensitivity and resolution needed for multicolor applications.

The BD FACSAria II cell sorter advances cell sorting with multiple improvements. A new fluidics design improves aseptic setup and cleaning. New nozzle sizes enable a wide range of particles to be sorted. Software improvements dramatically cut the time spent on cytometer setup, adjustment, and maintenance.
The BD FACSAria II cell sorter also sets a new standard for ease of use to improve both workflow and productivity. A new nozzle design allows for reproducible insertions. A new universal sort tube collection mount makes it easier to insert and remove tube holders. Software wizards and automation simplify aseptic sort setup, drop-delay determination, and clog detection. Compatibility with BD digital analyzers makes it easier to move from analysis to cell sorting."

The Agilent Human Genome CGH Microarray is a high-resolution tool for genome-wide DNA copy number variation profiling without amplification or complexity reduction. Comprehensive probe coverage is enhanced with emphasis on known genes, promoters, miRNAs, pseudoautosomal, and telomeric regions.

Agilent SurePrint G3 and High-definition CNV microarrays enable multiple loci to be assessed simultaneously, allowing for their identification and characterization. The microarrays provide high-resolution and optimized probe design for maximal sensitivity and specificity as required by the researcher.
Human High Resolution CNV microarray, 1x1M

The Agilent SurePrint G3 Human Comparative Genomic Hybridization (CGH) platform has been extended to include a set of Single Nucleotide Polymorphism (SNP) probes on the same microarray. This allows for the simultaneous, high-resolution detection of copy number and copy-neutral variations, without running two separate microarray experiments. The same straight-forward Agilent CGH workflow is used to prepare the samples for hybridization to the SurePrint G3 Human CGH+SNP microarrays. Restriction digestion of genomic DNA allows genotyping of SNPs located in the enzymes’ recognition sites. After labeling and hybridization, CGH+SNP microarray data are analyzed using novel algorithms contained within Agilent’s Genomic Workbench software.
Please put correct technique for these. Not sure what that should be.

The Agilent Human CpG Island Microarray provides researchers with comprehensive, genome-wide insight into how DNA methylation changes may be correlated to critical biological development processes including cancer, genomic imprinting, gene silencing and chromatin stability. Probes optimized for maximum sensitivity and specificity.
Human DNA Methylation microarray, 1x244k

The Agilent Human DNA Methylation Microarray is designed to interrogate known CpG islands and two additional groups of methylated DNA. The array is specifically designed for analysis of methylated DNA derived from affinity-based isolation methods such as methylated DNA immunoprecipitation (MDIP).

Agilent Human microRNA Microarrays generate complete microRNA profiles in order to provide broad insight into human expression and regulation. Probe design, coupled with a unique labeling method, works in concert to provide the optimal sensitivity and specificity that your work demands.
Human miRNA microarray Release 16.0, 8x60K

The Human ENCODE ChIP-on-chip Microarray is optimized using probes specifically validated for ChIP-on-chip technology. It is designed to specifically identify human DNA binding proteins within the ENCODE regions through traditional chromatin immunoprecipitation (ChIP) pull-down coupled with powerful DNA microarray location analysis.
changed name from unreadable URL. MH. Paula- is there a manufacturer for this item?

RNA services offered by the Functional Genomics Core include:

* RNA amplification
* RNA isolation
* RNA labeling –Cyanine dye
* RNA purification
* RNA quality control assessment
* RNA quantitation

TJ: removed * RNA QC service because looks like a duplicate of quality control assessment

Service includes:

* miRNA amplification
* miRNA isolation
* miRNA labeling-cyanine dye
* miRNA purification
* miRNA quality assessment
* miRNA quantitation

Please make Michelle the contact for all of this cores services

Includes mouse whole genome microarrays, human whole genome microarrays, and rat whole genome microarrays.

Available microarray services:

* Hybridization
* Image Quality Control
* Scanning

RNA must be already extracted from human tissue or blood.

In-gel digestion protocol for protein gels for mass spectrometry.

In Gel Digestion Procedure
(3-20-2011 by Powell)

1. Excise the band as closely as possible with a clean scalpel blade. Be sure to use gloves and a clean surface for excision.
2. Place the gel piece into a clean eppendorf tube and immerse it in 25mM AmBic in 50% acetonitrile.
3. Repeat step 2 until the gel piece is destained (typically twice).
4. After removing the destain buffer place the tube in the speedvac to dry.
5. Add 100µl 50 mM AmBic and ensure that the pieces are completely covered
6. Remove a DTT one shot from the freezer and add 90µl of mass spec water (500mM stock)
7. Add 2ul of the DTT stock to the re-hydrated gel piece arid incubate at 56°C for at least 30 min.
8. Reconstitute a one shot IAA tube from the freezer and add 150µl of mass spec water (1M stock, protect from light exposure).
9. Add 2µl of the stock to the gel piece and incubate at room temp for at least 30 min.
10. Dilute a fresh vial of trypsin (vials should be removed from -80°C and any unused portion can be re-used within a week at -20°C) 8µl to 312µl 50 mM AmBic kept on ice.
11. Add 10µl diluted trypsin for visible Coomasie bands or 5µl for clearer areas.
12. Incubate at 37°C with shaking for 4 hours.
13. Add 50µl of 0.5% formic acid and incubate 10 min
14. Remove the fluid and store at 4°C
15. Add 100µl of 100% Acetonitrile to gel slice and incubate 10 min with shaking, remove fluid to new tube
16. Add 100µl of 100% Acetonitrile to gel slice and incubate 10 min with shaking, combine with previous extraction
17. Dry down the extractions then add the fluid from step 14 to reconstitute.
18. The supernatant can be stored at -20°C or directly analyzed.
===============================================================================================
Notes: AmBIC = Ammonium Bicarbonate, solvents are all mass spec grade, never go into reagent bottles with a pipette (especially acids) pour out a portion into a separate container and then pipette from the container.

"The Department of Pharmacology and Toxicology provides educational programs for medical students and graduate students in the biomedical sciences. Our department offers study and research in a variety of pharmacology and toxicology sub-discipline areas, including:
cardiovascular, ocular, biochemical, neural, molecular, immunological and environmental."

Morehouse School of Medicine
Department of Pharmacology and Toxicology
720 Westview Drive
Atlanta, GA 30310

Department of Pharmacology & Toxicology, Morehouse School of Medicine

Research in Dr Tosini’s laboratory focuses on the roles that melatonin and the circadian clock play in the regulation of retinal physiology and pathophysiology. We have a series of Transgenic mice in which the melatonin receptors have been removed (melatonin receptors KO).

"One key issue in understanding the function of the retinal clock is to localize which cells types are capable of circadian oscillation. Until very recently the vast majority of the experimental evidence suggested that, as observed in non-mammalian vertebrates, circadian clock functions in the retina were localized to the classical photoreceptors cells, rods ands cones). Such a view has been recently challenged by a series of studies suggesting that the inner layer neurons may also contain circadian clocks and by the demonstration that retina lacking photoreceptors still expresses circadian rhythms in several retinal function. Studies in my laboratory are now underway to develop new culture methods to isolate and culture different type of retinal neurons (photoreceptor, amacrine and ganglion cells) to address this important question, and to identify the mechanisms (neural and humoral) that are responsible for the communication of the information among the different cells. In contrast to the SCN (the master circadian pacemaker in the brain) the retina is a complete circadian system where photoreception and pacemaker functions and physiological output are present in the same tissue and most likely in the same cells (photoreceptors), and therefore such an organ represents an ideal experimental model to understand the mechanisms of circadian rhythms generation, entrainment outputs control and biological significance."

Morehouse School of Medicine
Department of Pharmacology & Toxicology
720 Westview Drive
Atlanta, GA 30310

Chairman, Department of Pharmacology & Toxicology
Director, Circadian Rhythms and Sleep Disorders Program

"Bio-Plex Manager software empowers even the most inexperienced user to simply and quickly achieve proficiency and deliver results."

C3H MT1−/− knock-out mice homozygous for the rd1 mutation, generously donated by Drs. Reppert and Weaver (University of Massachusetts Medical School), were back-crossed with C3H/f+/+ mice in which the rd1 mutation has been removed to produce C3H/f+/+MT1−/−.
OTTO KURATOR: converted value of property: http://purl.obolibrary.org/obo/ERO_0000578 to embedded instance. The literal value was: 25-30% ganglion cells die out by 18months of age

Removal of melatonin receptor type 1 increases intraocular pressure and retinal ganglion cells death in the mouse.

Melatonin modulates visual function and cell viability in the mouse retina via the MT1 melatonin receptor.

Synthetic peptide (KLH-coupled) corresponding to residues surrounding Tyr15 of human cdc2

Rabbit anti-human dimethyl-Histone H3 (Lys79)

KLH-conjugated synthetic peptide containing the sequence (...DFme2KTD...) where me2K corresponds to dimethyl-lysine at position 79 of histone H3

Santa Cruz Biotechnology catalog #: sc-25310

Recombinant fragment: (pS) conjugated to KLH

No detectable cross-reactivity with skeletal or non-muscle myosin.
Biomedical Technologies, Inc catalog #: BT-562

NV: There is an issue with the display for immunogenic material: http://jira.eagle-i.net:8080/browse/ONT-1653.

Rabbit anti-human B-Cell Associated Protein 37 kDa
Rabbit anti-human Repressor of Estrogen Activity

Epitope specific to REA immobilizd on solid support

His-tagged fusion protein corresponding to amino acid residues 88-299 of REA-1/BAP-37/prohibitin-related protein

Purified recombinant rat prohibitin protein

Synthetic peptide (KLH-coupled) corresponding to amino-terminal sequence of human C/EBPβ

Recognizes_species
Homo sapiens
Mus musculus
Rattus norvegicus

Recombinant fragment (human) (N-terminal). Porin 31HL from EBV-transformed human B-lymphocytes was used as the immunogen.

Fusion protein corresponding to amino acids 432-528 of HIF1 alpha

Recognizes_species
Bovine
Homo sapiens
Mus musculus
Rattus norvegicus

Santa Cruz Biotechnology catalog #: sc-7947

HSP 90β amino acids 610-723 at the C-terminus

raised against amino acids 610-723 mapping at the C-terminus of HSP 90β of human origin

Epitope within amino acid residues 1-20 of human, mouse and rat TBP. The specific epitope for this antibody is accessible when the C-terminal domain of TBP is removed or when TBP is complexed with DNA.

N-terminal region of human E2F1 (aa 1-89).

PDCD6IP programmed cell death 6 interacting protein

Hypoxia inducible factor 1, alpha subunit

Expresses firefly luciferace in all regions of endogenous period 2 expression

25-30% ganglion cells die out by 18months of age

The RCMI Gene Profiling Core provides a variety of services related to analysis of gene expression, which includes genome and transcriptome analysis. Laboratories in the Gene Profiling Core include Functional Genomics, Gene Variation and DNA Sequencing labs.

The DNA Sequencing Lab provides both DNA sequence and fragment analysis of a variety of DNA templates, including genomic DNA, PCR amplicons and plasmid or cosmid constructs. The lab uses capillary electrophoresis sequencing technology and is built around the ABI 3130xl Gene Analyzer (16-capillary) and associated software for DNA sequence analysis.

The Functional Genomics Lab provides microarray services for research investigators using glass slide technology platforms. We are an Agilent Certified Core facility and a member of the Association of Biomolecular Research Core Facilities (ABRF). Specifically we provide assistance, or full services, from RNA isolation to scanning hybridized microarrays and raw data extraction. In conjunction with the Biomedical Informatics Unit (BIU) at MSM, we provide full services in the analyses of ‘-omics’ datasets. We support all microarray products from Agilent as well as any other array company that utilizes uncoated glass slide technologies. We also provide consultative services for custom microarray design.

The Gene Variation Core Lab assists investigators in application of genomic technologies to population-based research. The strategic plan is to create a link between basic science, clinical investigation and population-based research. The central hypothesis is that health disparities are related in part to ethnicity-specific DNA variants in critical genes that influence the susceptibility to common diseases. Accordingly, the major objective of the lab is to enhance health disparities research capacity at the molecular genetic level based on cutting-edge genomic technologies.

For more information about Gene Profiling Core services contact the individuals listed here or click on the links to the affiliated labs.

Nerimiah Emmett. PhD
Functional Genomics Core Leader
Medical Education Building C-346
Morehouse School of Medicine
720 Westview Drive
Atlanta, GA 30310-1495
Telephone 404-752-1679
nemmett@msm.edu

William Roth, PhD
DNA Sequencing Core Leader
Hugh Gloster B-315C
Morehouse School of Medicine
720 Westview Drive
Atlanta, GA 30310-1495
Telephone 404-752-1947
wroth@msm.edu

Qing Song, PhD
qsong@msm.edu
Gene Variation Core Leader
Research Wing D-203
720 Westview Drive
Atlanta, GA 30310
Telephone 404-752-1845

The Luminex Laboratory has Bio-Plex 200 and MagPix multiplex plate readers. These instruments allow simultaneous analytical measurement of multiple protein biomarkers using bead-linked assays. This provides for exquisite sensitivity, specificity, and precise measurements of analytes in small sample volumes.

The lab also offers 2D Gel services. The lab houses a BioRad automated spot picker (Proteome Works) and a BioRad gel imager (Molecular imagerFX). Bundled with this package is the latest version of PDQuest 8.0 for analysis of expression data from gels. Together this equipment allows us to offer services such as protein identification and analysis of protein modifications. This service complements LC/MS/MS analysis offered in the roteomics Lab.

Services include:
1. Multiplex Immunoassays
2. QuantiGene Multiplex Assays
3. 2D Gel analysis

Equipment/Technology
1. Luminex Bio-Plex 200, and MagPix readers
2. Bio-Plex Manager 6.0
3. BioRad 2D gel insruments
4. PD Quest 8.0

Service Fee
$ 200.00 per assay plate (Bioplex)
2D Gel Service by consultation

Morehouse School of Medicine
Luminex Service MEB C235
720 Westview Drive, SW
Atlanta, GA 30310

Research Core Facility, Room C-235, Medical Education Building
720 Westview Drive, SW
Atlanta, GA 30310

Physiology Dept.
720 Westview Dr. S.W.
Atlanta, GA 30310

The Zebrafish Lab offers the combination of low cost, rapid in vivo analysis and complex vertebrate biology. Zebrafish are closer to humans evolutionarily than yeast or insects or worms and experiments are faster and less costly than those using mice. This model system supports research in many research areas, including molecular genetics of cardiac development, heart disease, neurogenetics, cell signaling pathways, and genetics of cancer prevention, and is an excellent teaching tool to support research training programs.

Service Charges:

The Zebrafish Lab will provide consultative and fee for services to basic, translational and clinical investigators; for example, animal husbandry, embryo DNA microinjection, generation of transgenic models, morpholino knockdown, in vivo chemical screening strategies, and other custom services, as needed. All services are charged on an hour-by-hour consultation basis. For more information, please contact the core leader directly.

Room D-210, Research Wing
720 Westview Dr, SW
Atlanta GA 30310-1495

Room C-223, Medical Education Building
720 Westview Dr, SW
Atlanta GA 30310-1495

The Biological Manipulation Core offers model systems for manipulation and analysis of gene expression. Laboratories within this core include:

A. Microvesicle Lab

This Lab offers technical expertise in isolation and purification of Microvesicles, and in their analysis. This includes expertise in Microvesicle isolation and analysis. Further, this group has developed technology that allows targeted Loading of proteins into micro-vesicles. Microparticles are a very hot current topic, and this biotechnology has cross research focus area potential for both research and therapeutic uses in AIDS/ID, Cancer/Reproduction, Cardiovascular/Metabolic, and Neuroscience. This research area at MSM was originally funded through the HIV group, but quickly was turned into a collaborative, transdisciplinary funded research project through the RCMI T1 ARRA Project which brought together diverse researchers from AIDS/ID, Cancer, Cardiovascular/Metabolic focus areas together into a interacting group. This research area can be a collaborative, transdiciplinary research driver at MSM and across the RTRN.

B. Stem Cell Lab

The Stem Cell Lab offers consultative service to investigators interested in adult or embryonic stem cell research including induced pluripotent stem cells (iPSCs) from human and various animals. In addition, the lab offers technical expertise for investigators interested in generating preliminary data for grant application or who are funded to perform stem cell work and who can provide reagents. This includes deriving and growing stem cells from bone marrow, adipose tissue, and umbilical cord, growing commercially available adult embryonic stem cells and iPSCs, reprogramming adult tissue cells into pluripotent cells, and differentiating stem cells into various lineages. The lab is constantly developing new techniques that will benefit the research community. Currently, the focus is on establishing techniques for genome editing in iPSCs with the goal of generating human models of human genetic diseases for translation research.

C. Zebrafish Lab

The Zebrafish Lab offers the combination of low cost, rapid in vivo analysis and complex vertebrate biology. Zebrafish are closer to humans evolutionarily than yeast or insects or worms and experiments are faster and less costly than those using mice. This model system supports research in many research areas, including molecular genetics of cardiac development, heart disease, neurogenetics, cell signaling pathways, and genetics of cancer prevention, and is an excellent teaching tool to support research training programs.

The Zebrafish Lab will provide consultative and fee for services to basic, translational and clinical investigators; for example, animal husbandry, embryo DNA microinjection, generation of transgenic models, morpholino knockdown, in vivo chemical screening strategies, and other custom services, as needed. All services are charged on an hour-by-hour consultation basis. For more information, please contact the core leader directly.

This Lab offers technical expertise in isolation and purification of Microvesicles, and in their analysis. Technical Expertise: Dr. Ming Bo Huang, M.D. has formulated expertise through ongoing research in the MSM HIV Nef Pathogenesis Group. Dr. Huang has developed expertise in Microvesicle isolation and analysis. Further, this group has developed technology that allows targeted Loading of proteins into micro-vesicles. Microparticles are a very hot current topic, and this biotechnology has cross research focus area potential for both research and therapeutic uses in AIDS/ID, Cancer/Reproduction, Cardiovascular/Metabolic, and Neuroscience. This research area at MSM was originally funded through the HIV group, but quickly was turned into a collaborative, transdisciplinary funded research project through the RCMI T1 ARRA Project which brought together diverse researchers from AIDS/ID, Cancer, Cardiovascular/Metabolic focus areas together into a interacting group. This research area can be a collaborative, transdiciplinary research driver at MSM and across the RTRN.

Morehouse School of Medicine
Microbiology, Biochemistry and Immunology
HG Building Room B-319/B-321
720 Westview Dr. SW
Atlanta, GA 30310

Medical Education Building Room C-212
Morehouse School of Medicine
720 Westview Dr. SW
Atlanta, GA 30310

Dr. Huang is Research Instructor in the Department of Microbiology, Biochemistry and Immunology, and is director of the Microvesicle Lab in the Biological Manipulation Core.

Room C-321, Gloster Building
Microbiology, Biochemistry and Immunology
Morehouse School of Medicine
720 Westview Dr SW
Atlanta GA 30310-1495

The Stem Cell Lab offers consultative service to investigators interested in adult or embryonic stem cell research including induced pluripotent stem cells (iPSCs) from human and various animals. In addition, the lab offers technical expertise for
investigators interested in generating preliminary data for grant application or who are funded to perform stem cell work and who can provide reagents. This includes deriving and growing stem cells from bone marrow, adipose tissue, and umbilical cord, growing commercially available adult embryonic stem cells and iPSCs, reprogramming adult tissue cells into pluripotent cells, and differentiating stem cells into various lineages. The lab is constantly developing new techniques that will benefit the research community. Currently, the focus is on establishing techniques for genome editing in iPSCs with the goal of generating human models of human genetic diseases for translation research.

Room D-204, Research Wing
Morehouse School of Medicine
720 Westview Dr, SW
Atlanta GA 30310-1495

Room C-205, Medical Education Building
Morehouse School of Medicine
720 Westview Dr, SW
Atlanta GA 30310-1495

Ms. Jane Chu is Research Assistant in the Luminex lab, Analytical Chemistry and Protein Profiling Core (ACPP).

The specific objectives of the Study Design, Biostatistics, and Data Management Core are:

1: To provide consultation in study conceptualization and design, sampling, and sample size planning, statistical analyses planning, statistical analyses methodologies, and epidemiologic principles and methods.
2: To provide biostatistical analysis and data analytical support
3: To provide technical support and consultation in data collection methods, data entry, processing, and management.
4: To provide database administration support in creating and managing databases as shared resources

Services provided by the SDBDMC include:
Power and sample size calculations, Statistical analysis, Proposal development, Program evaluation, Evaluation plan development, Manuscript preparation, Study design, Survey development, Methodology development, Focus group moderation, Qualitative analysis, Data collection form development, and Database development and management.

We have streamlined the assistance request process by developing a web-based research assistance request form. The automated process captures more specifically the type of requests by faculty, staff, and students and allows these "customers" throughout MSM's campus and off site locations easier access the SDBDMC. Click the website link below for assistance.

Clinical Research Center
Morehouse School of Medicine, 720 Westview Dr., SW, Atlanta GA 30310-1495

Study Design, Biostatistics, and Data Management Core (SDBDMC)

Beckman Coulter Avanti J-26XPI Centrifuge

Specimen Coater for Scanning Electron Imaging

Equipment for cutting and trimming sections from cured resin blocks

Equipment for drying biological specimens for Scanning Electron Imaging

Cell Culture Supernatant, Plasma, Urine; Microparticles and Exosomeses.

Major Equipment
1. Beckman Allegra X-15R Tabletop Centrifuge (Rotor: FX6100)
2. Beckman Optima TLX Ultracentrifuge (Rotors: TLA-100.4, TLA-120.2)
3. Beckman Optima Max-XP Ultracentrifuge (Rotors: TLA-100.4, TLA-120.2)
4. Beckman L8M-80 Ultracentrifuge (Rotors: 50.2Ti, 42.1 Ti)

Special Equipment:
Nanosight LM10-HSBF Nanoparticle Characterization System.

Introduction

I. Basic Principles
Within a specially designed and constructed laser illumination device mounted under a microscope objective, particles in the liquid sample which pass through a beam path are seen by the instrument as small points of light moving rapidly under Brownian motion. The Microvesicle core Nanosight has the visual laser and measures (i) particle/vesicle size, and (ii) particle/vesicle number.

II. Nanoparticle Tracking Analysis (NTA) applied to the study of microvesicles
Microvesicles (100nm-1µm diameter) and exosomes (30 to 100 nm diameter) are small membrane vesicles secreted by most cell types, e.g. normal, diseased, and neoplastic cells and are present in blood and other bodily fluids. They contain a variety of molecules including signal peptides, mRNA, microRNA. It is significance in the development of vaccines, in targeting tumors, and in diagnosis and/or early detection. These microvesicles are a very hot current topic, and this biotechnology has cross research focus area potential for both research and therapeutic uses in AIDS/ID, Cancer/Reproduction, Cardiovascular/Metabolic, and Neuroscience.

Nanosight LM10-HSBF Nanoparticle Characterization System

Services Offered:
1. Isolation and Purification Microparticle or Exosome from Cell Cultures.
2. Isolation and Purification Microparticle or Exosome from Plasma.
3. Isolation and Purification Micropartical or Exosome from Urine.
4. Microparticle or Exosome samples

Data Analysis:
1. Microparticle or exosomes pictures (including 3D intensity image)
2. Microparticle or exosomes video (10-90 seconds/sample)
3. Microparticle or exosomes numbers (particle/ml)
4. Microparticle or exosomes size (10nM-1µM)

Associate Professor, Department of Community Health & Preventive Medicine Director of Biomedical Informatics Unit Associate Director, Master of Science in Clinical Research Program Director, T32 Interdisciplinary Research Training Program in Sleep and Cardiovascular Disorders

Research Assistant Professor/ Database Administrator

720 Westview Drive S.W.
Atlanta, GA 30310

Ernest Alema-Mensah, PhD, D.Min, MS, M.Div.

"The Cell and Tissue Imaging Facility provides a broad range of services covering many of the major structural methods used to study cells and tissues. The Core consists of four labs: Electron Microscopy, Flow Cytometry and Cell Sorting, Image Analysis, and
Molecular Histology."

The Electron Microscopy Lab provides complete scanning and transmission services from specimen fixation and dehydration, to examination and documentation via darkroom photography.

The Flow CytometryFACS, and Magnetic-bead Cell-sorting Lab houses a BD FACS Aria II and Miltenyi Biotec autoMACS Pro Seperator. Certfied BD- and Miltenyi-trained staff are available for consultation, acquiring data, or sorting cells.

The Image Analysis Lab provides a wide variety of options for the analysis and quantification of fluorescent probes, markers and antibodies within fixed and living cells. This lab houses a Leica TCS SP5 Confocal microscope, A DeltaVision Core Deconvolution microscope, a Tecan plate reader and a Zeiss Axioplan upright fluorescent microscope.

The Molecular Histology Lab is equipped to provide the following services: paraffin embedding, paraffin sections, frozen sections, immunohisto-chemical and H&E staining, and in situ hybridization."

For more information and service charges, contact:
Dr. James Lillard
Flow Cytometry and Cell Sorting Core Leader
Email: jlillard@msm.edu
Phone: (404) 752-1863

Dr. Woo-Kuen Lo
Electron Microscopy Core Leader
Email: wlo@msm.edu
Phone: (404) 752-1558

Dr. John Patrickson
Molecular Histology Core Leader
Email: jpatrickson@msm.edu
Phone: (404) 752-5717

Dr. Xuebiao Yao
Confocal & Fluorescent Imaging Core Leader
Email: xyao@msm.edu
Phone: (404) 752-1894

Currently consists of several rack/blade servers, each equipped with increased memory (96 GB) and hard drive space (18Tb) to house various biomedical/clinical applications capable of 40Gbps data transferring speeds. The servers are running Linux in conjunction with Kernel Based Virtual Machines (KVM) housing several virtual instances of biomedical/clinical databases and applications. A storage solution configuration guaranteeing high availability (HA) of data using a pair of NFS gateway servers in an active-passive configuration to provide resilient data access. The goal is to improve service availability and data integrity in the presence of possible failures or faults, and to maximize performance in the failure-free case. The NFS gateway servers connect multiple storage arrays (231TBs) to provide data access to the virtual machine instances created for clinical databases that are connected to application servers at 40Gbps speed to reduce latency. A high-performance computing (HPC) cluster was built with specialized components providing a fast, reliable, and scalable HPC solution to support processor intensive research. The HPC cluster is equipped with 180 cores and 1.4Tb of Random Access Memory (RAM).

We offer RNA isolation from cultured cells and tissues.

We measure the integrity of total RNA samples using the Agilent Bioanalyzer. The Agilent Bioanalyzer uses a micro channel based electrophoretic cell that allows rapid and sensitive investigation of nucleic acids.

Dept of Physiology
720 Westview Drive SW
Atlanta, Ga 30310

720 Westview Dr. SW
Atlanta, GA 30310-1495

Director, Laboratory Services and Research Resources

Our primary research goal is to study the role of cytochrome P450 (CYP) metabolites in the regulation of vascular and renal function. Obesity and type 2 diabetes not only cause metabolic abnormalities but also markedly affect vascular function, in particular endothelium-dependent agonist-induced vasorelaxation. Prostacyclin, nitric oxide (NO) and endothelium-derived hyperpolarizing factor (EDHF) are the main contributors to the endothelium-dependent vasodilation. Arachidonic acid CYP epoxygenase metabolites, epoxyeicosatrienoic acids (EETs), are potent vasodilators and they are EDHFs in the renal and mesenteric microcirculations. We have recently demonstrated that obesity and type 2 diabetes are associated with a decrease in CYP enzymes in the renal and mesenteric arteries. We are currently examining the regulatory mechanism and functional consequence of CYP metabolites in the development and progression of vascular and renal dysfunction in obesity and diabetes.
Aging is another important cardiovascular risk factor. Both morbidity and mortality are dramatically increased when aging process is associated with hypertension and obesity, a frequent pathology in the elderly population. Vascular aging is also mainly characterized by an impaired endothelium-dependent relaxation. A recent study has suggested that not only a defect in the NO pathway, but also a loss of EDHF-mediated responses may be responsible for impaired vasodilation in the aging kidney. We are also interested in studying the specific contribution of CYP metabolites to the regulation of endothelial function in aged kidneys.

Laboratory technician in the Stem Cell Laboratory, Biological Manipulation Core

Research Wing Room D-217
Cardiovascular Research Institute
Morehouse School of Medicine
720 Westview Dr, SW
Atlanta GA 30310-1495

Dr. Daniels is an epidemiologist/sociologist with expertise in study design and methodology, statistical analysis (both qualitative and quantitative), survey development, focus group facilitation, GIS, community engagement, program evaluation, and community intervention planning and implementation. Her primary research interests are in how preexisting social vectors (i.e., race, class, and gender) intersect to increase health disparities in minority populations and the effectiveness of motivational interviewing to reduce the incidence and prevalence of chronic disease; improve health outcomes; promote health behavioral change, and increase health literacy.

Clinical Research Center
Morehouse School of Medicine,
720 Westview Dr., SW,
Atlanta GA 30310-1495

Dr. Mayberry is Director of the Biostatistics, Study Design, and Data Management Core, Research Center for Clinical and Translational Sciences (R-CENTER), Morehouse School of Medicine. He is also Professor of Epidemiology), Department of Community Health and Preventive Medicine; Associate Director, Clinical Research Center; and Associate Director, ACTSI (Atlanta Clinical and Translational Sciences) Community Engagement and Research Program. He is the former Director of Health Equity Research, Institute for Health Care Research and Improvement, at Baylor Health Care System (BHCS), Dallas, Texas, and led efforts to better understand variations in care quality by race and ethnicity, socioeconomic status, and other patient characteristics; to improve healthcare quality by implementing evidence-based interventions to eliminate inequities in healthcare; and to integrate scientific findings into clinical decisions and healthcare policy to achieve equitable best care practices.
Dr. Mayberry is the former Director of the Program for Healthcare Effectiveness Research, at Morehouse School of Medicine (MSM), where he provided leadership in establishing an infrastructure of researchers, advisors, consultants, and technical research staff to support healthcare effectiveness, outcomes, health services, prevention, and disparities research. These efforts enhanced the infrastructure and current capacity of the MSM Clinical Research Center and the new R-CENTER.
Receiving his doctoral training in Epidemiology from the University of California at Berkeley, Dr. Mayberry has significant experience in research, teaching, program planning and evaluation, and policy analysis. He has served in several academic and governmental positions including the Assistant Dean of the Graduate School at the University of South Carolina and Senior Epidemiologist for Minority Health at the Centers for Disease Control and Prevention. He is active in local, state, and national health services and public health organizations including the Academy Health (nationally elected member of the Board of Directors and member of Annual Research Meeting Planning Committee), Coalition for Health Services Research, and the American Public Health Association (Epidemiology and Medical Care Sections Planning Committees). An appointed member of Georgia Governor's Council on Maternal and Infant Health, Dr. Mayberry chaired its Evaluation and Strategy Workgroups which identified priorities and solutions for maternal and infant health among stakeholders throughout the state. Recently, he led the evaluation of the Pfizer Foundation Southern HIV/AIDS Initiative and provided technical assistance to the 24 community-based organizations providing health education and prevention interventions to diverse local communities in nine states.
Dr. Mayberry is a nationally noted scientist, scholar, and an opinion leader in minority health and health disparities research. He has influenced policy and funding decisions for the Centers for Disease Control and Prevention, the Agency for Health Care Research and Policy, and the National Quality Forum in this regard. Dr. Mayberry serves as an expert consultant and scientific reviewer for several academic, private and federal organizations and reviewer for many peer-review scientific journals.

Dept. Community Health and Preventive Medicine
Morehouse School of Medicine,
720 Westview Dr., SW,
Atlanta GA 30310-1495

Dr. Yoo has extensive range of both collaborative and methodological research experience in health science and medical research. His research is mainly focused on developing biostatistical tools and methodologies that utilize complex and large amount of data to predict the risk of complex human diseases such as Cancer, Cardiovascular Diseases, Diabetes, and Mental disorders. Dr. Yoo is a noted scientist and scholar with many publications in epigenomics, clinical research in cardiovascular disease and cancer, statistical modeling on prediction and early detection, meta-analysis and prediction modeling using scoring system. His research interests include EpiGenomics approach to assess the effects of multiple polymorphisms, Nonparametric data-mining methods for detecting SNPs interactions, Statistical modeling for longitudinal biomarkers on early detection, Genetic risk scores based on patterns of polymorphisms and Meta-analysis.

MRC 509
Dept Community Health and Preventive Medicine
Morehouse School of Medicine,
720 Westview Dr., SW,
Atlanta GA 30310-1495

Associate Professor, Biostatistics, Dept. CHPM

Dr. Yan is an assistant professor in Biostatistics with strong knowledge of medical science and excellent statistical analysis skill, with expertise in statistical analysis for both survey data and clinical laboratory-based data. Areas of interest include experimental study design, the application of multivariate and hierarchical statistical modeling and database management.

The overall goal of the Morehouse School of Medicine R-CENTER clinical/translational research web portal is to integrate clinical and translational research information, data and knowledge resources, and provide a single point of entry for researchers across MSM and outside collaborations. The R-CENTER web portal also securely links to both internal and external Clinical and Translational Data Repositories such as i2b2 for collaborative and larger scale research projects and communicates internally and across collaborative research sites. The web-portal provides a secured interface for participants involved in the eHealthyStrides (Microsoft HealthVault project) to interact with the health vault data repository
1. An intuitive user interface where investigators can quickly and easily browse for information
2. A search engine for accurate and precise searches across MSM’s resources
3. Portlet technology that follows national standards (such as JSR 286) that will allow integration into applications and databases
4. Role-based authentication and authorization system to ensure only appropriate individuals have access to the appropriate resources
5. Federation-based authorization (pending) to facilitate collaboration across multiple institutions and work within a multi-institutional consortium

Research Center for Clinical and Translational Sciences (R-CENTER)

fluorescence activated cell sorting (FACS)

two dimensional polyacrylamide gel electrophoresis

journal article

curator note

mass spectrometer

assay

protocol

polymerase chain reaction

genotyping assay

gas chromatography detector

high performance liquid chromatography instrument

centrifugation

polymerization

flow cytometer analyzer

argon ion laser

helium neon ion laser

light source

flow cytometer sorter

DNA sequencer

array scanner

centrifuge

hybridization chamber

spectrophotometer

microarray

microtome

collecting specimen from organism

maintaining cell culture

concentrate

located_in

Academic Department

Center

Core Laboratory

Institute

Laboratory