NV: Accidentally clicked the "availability restricted to providing organization" checkbox. Not sure if it is true or false.
Experiment design and data analysis for Gene transcription microarray experiments.
Director, Molecular Biology Core Facility
protocol
"The PT Module is designed to simultaneously perform Dewaxing and Epitope Recovery on slides prior to immunostaining. Slides are loaded into the Autostainer racks which fit directly into the PT Module. The unit has an integrated computer with touch screen for programming a standardized Pre-treatment programs. The user may also modify the time and temperature settings to further optimize the results. The PT Module has dual reaction baths so that two individual procedures, each with 24 slides, may be run independently. LAB VISION'S proprietary Epitope Recovery solutions provide for safe, solvent-free dewaxing and improved environmental conditions."
NV: added type and removed term request.
gamma counter
has_related_disease
microtome
located_in
1005 Dr. D.B. Todd Jr. Blvd WBS 3109 Nashville,TN 37208
Core Laboratory
water bath
Harold D. West Basic Sciences Building 3106 1005 D. B. Todd Blvd. Nashville, TN 37208
Bio-Rad xMark Microplate Spectrophotometer
The xMark has the capability to perform standard colormetric ELISA and kinetic readings for general cell screening with 6, 12, 24, 96, 384, and 1536-well microplate formats. The xMark has a wavelength range of 200–1000 nm. xMark also has an optional incubation feature with a temperature range up to 45°C.
"Cytoscape is an open source bioinformatics software platform for visualizing molecular interaction networks and biological pathways and integrating these networks with annotations, gene expression profiles and other state data." Systems biology analysis software. Uses Java.
"Whenever possible, data analysis is approached as a training opportunity. However, there are times when this is undesirable either due to the complexity of the data (and analysis options) or the wishes of the investigator. In these instances, we do perform data analysis but request the participation of the investigator (particularly in the case of microarray data) as a fundamental understanding of the biology and experiment that can only be supplied by the person who has done the work is required for an appropriate analysis."
The Biolanalyzer determines the quantity and quality of DNA and RNA samples.
mass spectrometry data analysis objective
"The patented Para Trimmer is a one-of-a-kind innovation that saves time and reduces hassle, no scraping of cassettes is required. Multiple blocks may be passed along heated surface with ridges at one time and wax waste is collected for easy disposal."
"Dr. Goodwin oversees the operation of the Core facility. His expertise is in quantitative, live-cell imaging; however, he is knowledgeable in all aspects of the Core. He is available for consultation on projects and planning of experiments, and is willing to collaborate with investigators, if appropriate. He also conducts training on Core equipment for independent use."
3109 West Basic Sciences 1005 Dr. D.B. Todd Jr. Blvd. Nashville, Tennessee 37208
"Fixation Protocol For Processing And Parffin Embedding: • Samples submitted for processing MUST be properly trimmed (not to exceed 5 mm in thickness), and fixed in reagent of choice according to your protocol. However the most commonly used fixative is 10% Neutral Buffered Formalin (NBF). The laboratory keeps 10% NBF, sponges, and cassettes available. Samples that have been properly fixed should be transferred into 70% ethanol alcohol and taken to the laboratory for further processing. Please call the histology staff if you need guidance in these procedures. •All submitted tissue should he placed in cassettes and properly labeled with a unique ID number assigned by you. Please do not fill the cassettes to capacity with tissue. This practice will result in poor tissue processing. •Cassettes should be labeled and identified using the following system: JD08-####-tissue type. Where JD represents the first letter of the first and last name of the investigator, 00 represents the last two digits of the year, and #### is the unique ID number given by the investigator along with the tissue that is in the cassette. An example: for Jane Doe in the year 2007, 253 is the investigators unique ID number assigned by him/her and the tissue submitted is ovary. Would be: JD08-253-Ovary •Use a soft #2 pencil or Secureline Marker II/ Superfrost to label the cassettes. Do Not Use Sharpie Markers. The ink can’t with stand the chemicals used in processing. •For projects that require specially coated slides, please provide these slides at the submission of your project to the histology laboratory. Histology provides slides for routine hematoxylin and eosin (H&E) and IHC staining only. •Clearly identify all tissue containers submitted and all slides provided with investigator’s submitter’s name. •Please give one week prior notification for submissions that have a large number of samples or which will necessitate frequent and continuous submissions to the histology lab."
Fixation protocol for processing and paraffin embedding
curator note
1005 Dr. D.B. Todd Jr. Blvd. Nashville, Tennessee 37208
"The Endocrine Core Laboratory is a fully accredited, CLIA approved, endocrine testing facility funded by the Meharry Medical College Clinical Research Center, U54 Reproductive Health Grants, and institutional resources. The Endocrine Core Lab performs a comprehensive list of routine and specialized endocrine tests on blood samples for government funded investigators... A full staff of board certified clinical endocrinologists and clinical scientists are available for consultation and interpretation of test results."
complimentary analysis with molecular core
5029 Old Hospital Building 5th Floor 1005 Dr. D. B. Todd Blvd Nashville, TN 37208
Antibodies can be provided by the core laboratory for an additional charge.
Human Tissue Shared Resources Histology and Immunohistochemisty Core Protocols Histology and Immunohistochemisty Request Form: This is a fee for service laboratory. In order to gain any access to the cores services an account registration form must be completed along with a service request form. Both forms can be completed online or picked up in room: 3121 located on the 3rd floor of the Harold D. West Basic Sciences Building. The PI and the foapal account number to be charged must be completed. Also please provide us with the most accurate information requested on all forms. Incorrect or incomplete forms will delay the completion of your project. Tissue Submission: The submitting lab performs dissection, grossing and fixation if required. Animal tissue that is being submitted for processing should have all hair removed. However, the core will perform these procedures by request only. Submit tissues in cassettes, blocks or slides with the appropriate labeling, an example is given in the fixation section. If cassettes are submitted, please note the container solution (Ex. Fixative, alcohol) and preferred processing protocol (15min, 30min or 1hr per station). Fixed specimens, unless otherwise noted, are assumed to be at their end-point of fixation. Specimens for frozen sectiong are to be frozen already at -80° C. Frozen samples should be handled immediately (unless in storage at -80° C) so scheduling with the core tech MUST be reserved in advance. All projects submitted to the core facility are completed in the order received, using the submitted date indicated on the submission form. •All submissions should he taken to room: 3121 Located on the 3rd floor of the West Basic Science Building and handed to the core tech. You should have an approval number accompanying the submitted specimen once your request has been received and approved. Fixation Protocol For Processing And Parffin Embedding: •Samples submitted for processing MUST be properly trimmed (not to exceed 5 mm in thickness), and fixed in reagent of choice according to your protocol. However the most commonly used fixative is 10% Neutral Buffered Formalin (NBF). The laboratory keeps 10% NBF, sponges, and cassettes available. Samples that have been properly fixed should be transferred into 70% ethanol alcohol and taken to the laboratory for further processing. Please call the histology staff if you need guidance in these procedures. •All submitted tissue should he placed in cassettes and properly labeled with a unique ID number assigned by you. Please do not fill the cassettes to capacity with tissue. This practice will result in poor tissue processing. •Cassettes should be labeled and identified using the following system: JD08-####-tissue type. Where JD represents the first letter of the first and last name of the investigator, 00 represents the last two digits of the year, and #### is the unique ID number given by the investigator along with the tissue that is in the cassette. An example: for Jane Doe in the year 2007, 253 is the investigators unique ID number assigned by him/her and the tissue submitted is ovary. Would be: JD08-253-Ovary •Use a soft #2 pencil or Secureline Marker II/ Superfrost to label the cassettes. Do Not Use Sharpie Markers. The ink can’t with stand the chemicals used in processing. •For projects that require specially coated slides, please provide these slides at the submission of your project to the histology laboratory. Histology provides slides for routine hematoxylin and eosin (H&E) and IHC staining only. •Clearly identify all tissue containers submitted and all slides provided with investigator’s submitter’s name. •Please give one week prior notification for submissions that have a large number of samples or which will necessitate frequent and continuous submissions to the histology lab. Frozen Section Protocols: Samples submitted for frozen sections are required to be in clamshell molds or cryo-molds. The laboratory keeps clamshell molds and is available by request only. •Place a small amount of OCT in the mold and set on dry ice to slightly cool. OCT will begin to thicken and turn hazy, add the sample and fill/ cover with OCT. •Label the molds according the example given for cassette labeling. •Place molds on dry ice and transport to the lab to be stored in -80° C freezer until it is cut. •You should contact the core staff in advance for all frozen sections that are going to be performed. To ensure proper handling of all samples. •Some antibodies for IHC stains require frozen sections; please contact the core tech to inquire about these particular antibodies. Human Tissue Acquisition Shared Resource Tissue Procurement: In order for the core to collect and provide human research material, each investigator is required to complete a tissue procurement request form. The investigator should indicate the tissue type and amount of tissue needed, describe tissue storage and transfer conditions (e.g., media, snap freezing, and sterility requirements) and specifies limiting factors in tissue collection (e.g. age, sex, race, etc.).
associated with molecular core and proteomics core.
Human Tissue Shared Resources Histology and Immunohistochemisty Core Protocols Histology and Immunohistochemisty Request Form: This is a fee for service laboratory. In order to gain any access to the cores services an account registration form must be completed along with a service request form. Both forms can be completed online or picked up in room: 3121 located on the 3rd floor of the Harold D. West Basic Sciences Building. The PI and the foapal account number to be charged must be completed. Also please provide us with the most accurate information requested on all forms. Incorrect or incomplete forms will delay the completion of your project. Tissue Submission: The submitting lab performs dissection, grossing and fixation if required. Animal tissue that is being submitted for processing should have all hair removed. However, the core will perform these procedures by request only. Submit tissues in cassettes, blocks or slides with the appropriate labeling, an example is given in the fixation section. If cassettes are submitted, please note the container solution (Ex. Fixative, alcohol) and preferred processing protocol (15min, 30min or 1hr per station). Fixed specimens, unless otherwise noted, are assumed to be at their end-point of fixation. Specimens for frozen sectiong are to be frozen already at -80° C. Frozen samples should be handled immediately (unless in storage at -80° C) so scheduling with the core tech MUST be reserved in advance. All projects submitted to the core facility are completed in the order received, using the submitted date indicated on the submission form. •All submissions should he taken to room: 3121 Located on the 3rd floor of the West Basic Science Building and handed to the core tech. You should have an approval number accompanying the submitted specimen once your request has been received and approved. Fixation Protocol For Processing And Parffin Embedding: •Samples submitted for processing MUST be properly trimmed (not to exceed 5 mm in thickness), and fixed in reagent of choice according to your protocol. However the most commonly used fixative is 10% Neutral Buffered Formalin (NBF). The laboratory keeps 10% NBF, sponges, and cassettes available. Samples that have been properly fixed should be transferred into 70% ethanol alcohol and taken to the laboratory for further processing. Please call the histology staff if you need guidance in these procedures. •All submitted tissue should he placed in cassettes and properly labeled with a unique ID number assigned by you. Please do not fill the cassettes to capacity with tissue. This practice will result in poor tissue processing. •Cassettes should be labeled and identified using the following system: JD08-####-tissue type. Where JD represents the first letter of the first and last name of the investigator, 00 represents the last two digits of the year, and #### is the unique ID number given by the investigator along with the tissue that is in the cassette. An example: for Jane Doe in the year 2007, 253 is the investigators unique ID number assigned by him/her and the tissue submitted is ovary. Would be: JD08-253-Ovary •Use a soft #2 pencil or Secureline Marker II/ Superfrost to label the cassettes. Do Not Use Sharpie Markers. The ink can’t with stand the chemicals used in processing. •For projects that require specially coated slides, please provide these slides at the submission of your project to the histology laboratory. Histology provides slides for routine hematoxylin and eosin (H&E) and IHC staining only. •Clearly identify all tissue containers submitted and all slides provided with investigator’s submitter’s name. •Please give one week prior notification for submissions that have a large number of samples or which will necessitate frequent and continuous submissions to the histology lab. Frozen Section Protocols: Samples submitted for frozen sections are required to be in clamshell molds or cryo-molds. The laboratory keeps clamshell molds and is available by request only. •Place a small amount of OCT in the mold and set on dry ice to slightly cool. OCT will begin to thicken and turn hazy, add the sample and fill/ cover with OCT. •Label the molds according the example given for cassette labeling. •Place molds on dry ice and transport to the lab to be stored in -80° C freezer until it is cut. •You should contact the core staff in advance for all frozen sections that are going to be performed. To ensure proper handling of all samples. •Some antibodies for IHC stains require frozen sections; please contact the core tech to inquire about these particular antibodies. Human Tissue Acquisition Shared Resource Tissue Procurement: In order for the core to collect and provide human research material, each investigator is required to complete a tissue procurement request form. The investigator should indicate the tissue type and amount of tissue needed, describe tissue storage and transfer conditions (e.g., media, snap freezing, and sterility requirements) and specifies limiting factors in tissue collection (e.g. age, sex, race, etc.). Application Process: Investigators must submit a completed application. This application can be picked up at room: 3121 located in the West Basic Sciences Building or you can request for an application to be sent through e-mail. Once the application is received it will go through an approval process and you will be contacted in 2-3 business days. If any information need for completion of the application is missing, the application will be in pending status until we receive the necessary documents. Procurement for your project will not begin until that information is received. Please read over the application carefully before completing and turning it in. All fees are included on the application, if you are requiring any histological services to be performed please look at the core facility fees and services for prices. Agreement For Use Of The Core Facility And Its Tissue The recipient agrees to acknowledge the contributions of the Meharry Medical College-Human Tissue Acquisition Shared Resource Core in all publications resulting from the use of the lab and the tissues. Recommended wording to the methods or acknowledgement section is as follows: For tissue: “Tissue Samples were provided by the Meharry Medical College-Human Tissue Acquisition Shared Resource Core which is funded by U54 Cancer Grant/ National Cancer Institute. Other investigators may have received specimens from the same subject.” For histology and immunohistochemistry services: “All histology and immunohistochemistry services were provided by the Meharry Medical College-Human Tissue Acquisition Shared Resource Core which is funded by U54 Cancer Grant/ National Cancer Institute.”
"Collection of fresh tissue, snap freezing and banking neoplastic and normal tissues."
histological sample preparation
"* The Wizard is the latest and most advanced gamma counter. * The Wizard can operate as automatic standalone CPM, or it can be used to do extensive data evaluation with its own internal RiaCalc WIZ program or it can be linked up to an external PC and use the power of a MultiCalc program."
histology
Assays for the following hormones: Estradiol Follicle Stimulating Hormone (FSH) Insulin Luteinizing Hormone (LH) Progesterone Prolactin Sex Hormone Binding Globulin (SHBG) Total Testosterone Thyroid Stimulating Hormone (TSH) 17 α- OH Progesterone
We provide assistance with data analysis and acquiring images.
"The Ultra Pro 5000 series Cryostats offers the ultimate in precision and constant reproducibility in the preparation of frozen sections. Backed by years of cryostat technology, the UltraPro 5000 Cryostat includes the robust 5040 rotary retracting microtome. The Ultra Pro 5000 series has been proven in hospitals, universities and research departments cutting a diverse range of hard and soft tissues, plastics, rubber, bone, man-made fibres, wood and many other materials. With a wide range of optional features to choose from, the UltraPro 5000 can, justifiably, be called 'the complete cryostat'."
Center
Meharry Medical College 1005 Dr. D.B. Todd Boulevard Nashville, TN 37208
Pathology Professor and Department Chair
"Dr. Mohka ensures that the Core meets the needs of the SNRP program, the principal resource funding the Core, and is working toward anticipated future needs of Meharry investigators."
B47 Biomedical Center 1005 Dr. D.B. Todd Jr. Blvd. Nashville, Tennessee 37208
DNA sequencer
related_software
"Collection of fresh tissue, snap freezing and banking neoplastic and normal tissues, histology services such as paraffin embedding, paraffin sectioning, automated Hematoxylin and Eosin staining, frozen sectioning, special stains, and immunohistochemistry. Human and animal pathology diagnostic services and microscopic interpretation of Hematoxylin and Eosin, special stain, and immunohistochemistry interpretations, digital photography and descriptions for manuscripts are also available."
automated dewaxing and epitope recovery device
"MATLAB® is a high-level language and interactive environment that enables you to perform computationally intensive tasks faster than with traditional programming languages such as C, C++, and Fortran."
centrifugation
"Keeps water at a constant temperature to flatten wax or resin sections prior to lifting them onto a microscope slide. It may also be used for hot staining except with corrosive substance"
Circular Paraffin Section Flotation Bath
material processing
Tennessee Hemoglobinopathy Confirmatory Laboratory
The Hemoglobinopathy Confirmatory Laboratory for the State of TN Newborn Screening Program is housed at the MSCC, so all children born in the state of Tennessee who have screened positive for a hemoglobinopathy and all adults seeking to know their hemoglobinopathy status undergo state of the art evaluation at the MSCC Hemoglobinopathy Laboratory. High performance liquid chromatography, alkaline, acid, neutral and isoelectricfocusing electrophoresis are utilized to confirm or establish a hemoglobinopathy diagnosis.
"It is the mission of the Meharry Medical College Morphology Core to provide the expertise and state-of-the-art instrumentation necessary to facilitate and enhance the productivity of Meharry’s investigators and trainees in the areas of fluorescence and bright-field microscopy and immunohistochemistry. This core strives to accomplish this by offering consultation on instrumentation and experimental design, training on instrumentation, and collaboration opportunities. We do our utmost to stay on the cutting edge of technology, skills, and knowledge in the field."
"Our Proteomics Core aids investigators and trainees with high performance computation in proteomics, as well as other "omics" type applications needing efficient analysis of large-scale biological data sets. The ability to incorporate proteomics into translational and clinical biomedical research is critical for the discovery of therapeutic interventions and high fidelity biomarkers of disease and response to therapy. The Core provides state-of-the-art services, training, and bioinformatics-driven data analysis to the Meharry translational research community."
Nikon A1R confocal microscope: o Live cell imaging with stage top incubator o Perfect focus o Dual hybrid scanner for resonant (up to 430 images/s) and galvanic (high resolution) scanning o 6 laser lines 405 458 488 514 561 640 o Photobeaching capabilities for FRAP, FLIP, FRET, selective photobleaching, and photoconversion or photoactivation
3100 Genetic Analyzer is a multi-color fluorescence-based DNA analysis system using the proven technology of capillary electrophoresis with 4 capillaries. (ABI)
microscope
* This confocal has 488 nm, 543 nm, and 633 nm laser lines to excite fluorophores with compatible excitation characteristics. Fluorescence emission is collected with 505-550 nm, 560-615 nm, and 650 nm filter sets, respectively, in independent channels. The microscope is also equipped with a fourth detector to collect light for differential interference contrast (DIC) imaging. * There are 10x, 20x, 40x, 60x, and 100x objective lenses available for use on this microscope. They are all capable of imaging using DIC, making it possible to do brightfield confocal imaging. * Motorized z-plane movement allows for optical sectioning through a sample. The EZ C1 confocal software can be used subsequently to reconstruct serial z-plane images into a 3D rendering.
Nikon TE2000-U C1 confocal laser scanning microscope
centrifuge
The Olympus BX 50 stereology microscope provides a method to collect data on stained tissue to examine number, length, area, and volume using the Stereologer software. This microscope significantly reduces the time needed to quantify staining of tissue samples, while providing an accurate, unbiased measurement.
Confocal and wide-field microscopy training
"The TE2000-E wide-field microscope is equipped for diverse imaging: * Motorized z-focusing motor enables collection of in focus images in different image planes. This utility is most often used in acquiring images for deconvolution and Stereolgy. * It has a xenon lamp for fluorescence excitation provides an excellent light source for calcium imaging with Fura-2 and UV-Vis fluorophores. * A very sensitive CoolSNAP HQ2 camera is ideal for fluorescence imaging, as it produces very little noise and allows for faster integration times. * A 5 mega pixel color camera is available for non-fluorescent samples and is ideal for histological stains. * There are 4x and 10x phase lenses available as well as high NA 20x, 40x, 60x, and 100x DIC lenses. * A computer controlled excitation filter wheel allows for fast, automated collection of images. * Filter sets are available for UV, blue, green, and red excitation and emitted light is collected with the corresponding band pass or long pass emission filters. * Filter sets are available for calcium ratio imaging, specifically fura-2 AM. * A motorized stage in conjuction with Nikon NIS Elements or Stereologer software allows for the collection of images in multiple, specified locations. * Nikon NIS Elements Advanced Research imaging software is cable of 6 dimensions (6D) of acquisition. Up to six combinations of wavelength, time, x-, y-, and/or z-positions are possible to control with this software."
1005 Dr. D.B. Todd Jr. Boulevard Nashville, TN 37208 Metro General Hospital 2nd floor
Medical care is provided for affected adults and adolescents in the facilities of Metro Nashville General Hospital. These services include care provided for hospital admissions and clinic visits each year for adolescent and adult patients. Sickle cell clinic sessions are held on Monday afternoons.
"The Clinical Research Center (CRC) at Meharry Medical College is a joint project of the Minority Biomedical Research Support (MBRS), the General Clinical Research Centers (GCRC) Program of the Division of Research Resources, National Institutes of Health, and Research Centers in Minority Institutions Program (RCMI) of the National Center for Research Resources. The purpose of the CRC is to provide the resources necessary to facilitate clinical research in a minority academic setting by expanding existing programs, nurturing new investigators, and encouraging full utilization of its services. Our mission is to: 1) Promote the advancement of clinical research and improve the health and welfare of disadvantaged and minority populations through participation in clinical studies. 2) Promote an environment that encourages, stimulates, nurtures, and attracts clinical research by providing leadership and support for conducting clinical research."
NIS-Elements performs live cell, 6D experiments quickly and flawlessly. It can organize X, Y, Z, Lambda (wavelength) and Time within one integral platform, for easier-than-ever multi-dimensional imaging. Four types of “dimensions” of acquisition – Time, Multipoint, Z-series, Wavelength (Multichannel)— can be selected. NIS-Elements innovative analysis capabilities include object counting, automated measurements, region of intent measurements and time measurements including image ratioing of multi-channel images. Advanced visualization methods, like capturing 3D data sets, 2D/3D data set analysis, and large image stitching can all be performed with relative ease with the software. NIS-Elements offers a rich spectrum of mathematical morphology functions (clean, erode, dilate, open, close, smooth), morphologic separation functions, linear morphology functions, fill functions (fill holes, close holes), skeleton functions (medial axis, skeletonize, pruning) and other functions (such as: binary invert, convex hull, contour, skeletonize, homotopic marking, zones of influence, etc.) Multiple single channel images (captured with different optical filters or under different camera settings) can be merged together simply by dragging from one image to another. In addition, the combined images can be stored to a file while maintaining their original bit depths or optionally can be converted into a RGB image.
"The Meharry Sickle Cell Center (MSCC) has more than a thirty-five year history of service to the community and to patients with Sickle cell disease and other hemoglobinopathies. In this capacity, the Center provides comprehensive services including clinical care, education, genetic counseling, laboratory services, and research. The Center's Director, Steven N. Wolff, M.D., provides comprehensive medical care for adults with sickle cell disease residing in the 40 Counties in the Middle Tennessee area. The Center’s Associate Director and Laboratory Director, Maria del Pilar Aguinaga, PhD., DLM (ASCP), along with Robert Mushi, PhD, and Celestine Mgbemere, MD provide diagnostic services as members of the Hemoglobinopathy Confirmatory Laboratory for the State of TN Newborn Screening Program, which is housed at the MSCC. In this capacity, all children born in the state of Tennessee who have screened positive for a hemoglobinopathy and all adults seeking to know their hemoglobinopathy status undergo state of the art evaluation at the MSCC Hemoglobinopathy Laboratory. High performance liquid chromatography, alkaline, acid, neutral and isoelectricfocusing electrophoresis are utilized to confirm or establish a hemoglobinopathy diagnosis. Annually, over 6,000 blood samples for suspected hemoglobin disorders undergo laboratory evaluation at the MSCC. Health care agencies and parents of children identified with positive hemoglobinopathy results are promptly notified so that proper medical management can be instituted. Additionally, the MSCC provides follow-up services to newborns with sickle cell disease to ascertain that all children are undergoing appropriate preventive care such as receiving prophylactic antibiotics by two months of birth and their recommended vaccines. The Sickle cell clinic is located on the second floor of Metropolitan Nashville General Hospital. It provides easy access to the laboratory and other hospital resources. The Center's Follow-Up Nurse, Ms. Ronlanda Foley provides continuity of care and clinical follow-up to the adult and pediatric patients with sickle cell disease. Ms. Tené Hamilton Franklin provides genetic counseling and educational services to the families affected with sickle cell disease and other hemoglobinopathies. The MSCC sponsors monthly support group meetings for adults, adolescents and children with sickle cell disease. Adult Support Group meetings take place at Matthew Walker Comprehensive Health Center, Transition Support Group for adolescents and Pediatric Support Group for children with sickle cell disease, take place at the Pediatric Sickle Cell Clinic of Monroe Carrell Jr. Children’s Hospital at Vanderbilt. The MSCC organizes several community activities for patients, families and health care providers. During Sickle Cell and Pain Awareness Month (Month of September), the MSCC organizes an Annual Walk-A-Round Campus to raise awareness for Sickle Cell Disease, promote health and fitness, and acknowledge individuals in the community dealing with Sickle Cell or a family member caring for someone with Sickle Cell Disease. Other sickle cell month activities include hemoglobinopathy screenings; health exhibits, a blood drive for sickle cell patients; a heritage lecture on sickle cell disease in memory of the late Dr. Joseph Phillips, a former Meharry professor who was affected by sickle cell disease and numerous presentations and educational activities in the community. Currently under early development is a coordinated effort with Vanderbilt University to develop a regional comprehensive care center for patients with sickle cell disease. This center would combine resources from many institutions to develop an outpatient facility to provide medical and psychosocial care for children, their families and adults with hemoglobinopathies. The Middle Tennessee region should be proud of the heritage of the Meharry Sickle Cell Center as one of the first centers in the country and as a leading center for the care of patients with Sickle cell disease in our region."
1005 Dr. D.B. Todd Jr. Boulevard. Old Hospital Building Suite 501- D.
"* Moderate complexity automated instrument that has expandable test menu * Using chemiluminescence reagents * Has programmed standard curve set in each kit * Reagents/assay kit are specific to each kit lot * Kit adjustments needed every two weeks"
a one-dimensional nano HPLC configured with a pump and comes with an AB Sciex 920 Autosampler with cooling module for rapid injection of sample. The system uses Microfluidic Flow Control (MFC) that allows precise operation at pressures up to 5,500 psi and enables automated peak parking for extended MS/MS analysis of analytes of interest. Additionally, the Eksigent NanoLC-1Dplus system comes with a dedicated sample-loading pump that allows sample loading at higher flow rates, up to 30 uL/min, for significant timesavings and flexibility
NV: Removed term request for autostainer.
Laboratory
Meharry Medical College 1005 D. B. Todd Boulevard Nashville, TN 37208
Need to review with Nicole and for proper term usage
The Molecular Biology Core Facility at Meharry Medical College is a multi-functional facility that provides to faculty, students, and staff of the college the primary service of DNA sequencing but also provides access to shared equipment housed in a centralized location and facilitates convenient and rapid access to frequently used molecular biological reagents. The Molecular Biology Core houses a number of shared items of research instrumentation. The core also maintains an Invitrogen Supply Center that stocks many routinely used reagents for molecular biological research. The major service activities of the Molecular Biology Core are to provide training and technical assistance with the use of shared equipment housed in the core and provide convenient access to molecular biological reagents. Training in the proper use of equipment is provided by the Laboratory Coordinator and also as needed by vendor technical personnel. The core maintains in-house freezers and supply cabinets stocked with many commonly used molecular biological reagents from Invitrogen. In-house reagents are ordered online from Invitrogen and picked up from stocks housed in the core. Invitrogen items not stocked in the core can be ordered online without shipping charges.
" The Gel Logic 4000 PRO offers innovative and advanced functionality for quick capture of high resolution images of gels, blots and plates. A luminescent, UV-fluorescent and radioisotopic imaging system."
Software for operation and analysis of data for CFX96
Services include: * Staining/sample preparation * Sample data acquisition * Computer/dedicated software data analysis
Human Tissue Acquistion and Pathology Core
Harold D. West Basic Sciences Building 3101 1005 D. B. Todd Blvd. Nashville, TN 37208
"The Human Tissue & Pathology Core provides tissue collection and storage services and access to human tissue, histology services, and specialized equipment. The Core collects human tissues prospectively from routine surgical resections and autopsies for use in basic, translational, and clinical research. Only remnant tissue samples not needed for diagnostic purposes are collected. Tissues are available fresh, snap frozen, or in a variety of fixatives, and normal, neoplastic, and other diseased tissues are available. Histology services include paraffin embedding, paraffin sectioning, automated Hematoxylin and Eosin staining, frozen sectioning, special stains, immunohistochemistry and other specialized processing and sectioning services."
Human Tissue Acquistion, Histology and Immunohistochemistry Shared Resource
615-327-5714 1005 Dr. D.B. Todd Boulevard WBS 2203 Nashville, TN 37208
Old Hospital Building Meharry Medical College 1005 Dr. D. B. Todd Blvd Nashville, TN37208
"The FACS Core provides a state-of-the-art multi parameter flow cytometry and biosafty level 3 facility on the Meharry Medical College campus. The facility performs flow cytometry analysis, provides expertise in implementing and developing technologies for cell analysis, and provides training to investigators in the use of the instrumentation and in the interpretation of data analysis"
'Thermo Scientific Varistain Gemini ES Automated Slide Stainer is a multi-dimensional stainer designed to maximize efficiency by organizing staining workflow needs.'
Varistain Gemini ES Automated Slide Stainer
"Training in the proper use of equipment is provided by the Laboratory Coordinator and also as needed by vendor technical personnel."
Shared equipment training and technical assistance
Access to shared equipment and molecular biological reagents
"The core maintains in-house freezers and supply cabinets stocked with many commonly used molecular biological reagents from Invitrogen. In-house reagents are ordered online from Invitrogen and picked up from stocks housed in the core. Invitrogen items not stocked in the core can be ordered online without shipping charges. "
Confocal and wide-field microscopy services
Training on instrumentation is provided along with a detailed training user guide. We provide imaging assistance to collect fast and high quality images and data. Technical assistance is available 9am-5pm and other times upon request.
The CFX96 provides sensitive detection for precise quantitation and target discrimination. The optical system automatically collects data from all wells during data acquisition.
"The TC10 cell counter completes the counting of mammalian cells—suspended or re-suspended adherent—in one simple step, initiating a count immediately upon slide insertion. Unlike other cell counting devices, which may draw inaccurate conclusions from using a single plane of focus to assess cell viability, the TC10 cell counter scores each sample across multiple planes. The unique auto-focus technology and image analysis algorithm provide accurate and reproducible cell counts in less than 30 seconds for cell concentrations between 5 x 104 and 1 x 107 cells/ml and cells within a 6–50 µm diameter range. Since only 10 µl of suspended cells is required for counting, precious sample is conserved. Cell viability can also be assessed with the TC10 cell counter with the use of trypan blue, a dye which is excluded from live cells and incorporated into dead cells. The system recognizes the presence of trypan blue without user intervention and provides both cell count and cell viability, in less than 30 seconds."
"The Meharry Microarray and Bioinformatics Core (MMBC) provides state of the art training, instrumentation, services and bioinformatics-driven data analysis to the Meharry Community in microarray experimental design, manipulation, and gene expression analysis. We also aid investigators and trainees in validation of microarray results using RNA QA/QC and real time PCR results. Finally, this Core Resource aids Meharry investigators and trainees with high performance computation in proteomics as well as genomics. Gene and protein pathways analysis and shotgun proteomics (mass spectrometry) analysis are just two more of the services that we provide. It is our aim to keep this facility up to date computationally and intellectually with state of the art “Omics” services to support education and research in microarray, genomics and proteomics data analysis, computational biology, and systems biology."
1005 Dr. D.B. Todd, Jr. Boulevard Nashville, TN 37208 (615) 327-6111
coupled with a nanospray ionization source and provides MSn dissociation analysis. It is good for protein identification and shotgun proteomics analysis.
ThermoFinnigan LTQ Ion Trap Mass Spectrometer
Shandon Finesse ME+ Thermo Scientific Finesse ME+
Dr. Aguinaga received her Bachelor's and Licenciate degrees in Biology and her Master's degree in Biochemistry from Universidad Peruana Cayetano Heredia in Lima, Peru. She did post-graduate training at the Biophysics Laboratory of the University of Wisconsin-Madison, and received her Ph.D. degree in Biophysics/Molecular Biology from Kanazawa University Medical School in Kanazawa, Japan. She came to Meharry Medical College as a post-doctoral research associate and rose through the ranks to tenured Professor in the Department of Obstetrics and Gynecology and is the Associate Director and Laboratory Director of the Meharry Sickle Cell Center. Dr. Aguinaga holds an Adjunct Faculty appointment in the Department of Medicine, Division of Hematology/Oncology at Vanderbilt University. Dr. Aguinaga’s research interests are in sickle cell disease and women’s health and her clinical interest lies in the diagnosis of hemoglobin disorders, for which she is consulted nationally and internationally. She directs the State of TN Newborn Screening Hemoglobinopathy Confirmatory and Reference Laboratory housed at the Meharry Sickle Cell Center. She has numerous publications and presentations in the field of hemoglobinopathies. Her research work has been funded by NHLBI, NICHD, HRSA, OMH and the State of Tennessee Department of Health. Dr. Aguinaga serves in several departmental, institutional and national committees. She is the current president of the EE Just Hematology Society (Society of Minority Hematologists) and has received several honors and awards throughout her academic career. Dr. Aguinaga has served as ad-hoc grant reviewer for the National Heart, Lung and Blood Institute and is a reviewer for the American Society of Hematology and ad hoc editor for The American Journal of Hematology and the Journal of Healthcare for the Poor and Underserved. Dr. Aguinaga volunteers in the community with various community organizations serving special and underprivileged populations like, The YMCA-Latino Achievers Program (member and former Chair of the Board), The Center for Health Services at Vanderbilt University (member and former Vice-Chair of the Board), and the Nashville Task Force on Refugees and Immigrants (member). Dr. Aguinaga is certified by the American Society for Clinical Pathology Board of Certification as Diplomate in Laboratory Management, Medical Laboratory Scientist and Technologist in Molecular Biology. Dr. Aguinaga is licensed by the State of Tennessee as a Medical Laboratory Director.
AMG EVOS (fluorescence) Digital Inverted Microscope
spectrophotometer
This facility holds breast tissue from patients representing various ethnic backgrounds, age range, and stages. Services can be tailored to PI's request.
"The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells on a single platform. Results are delivered within 30-40 minutes in automated, high quality digital data."
The Excelsior ES Tissue Processor Features: An easy-to-use operating mode for routine operation. Systematic logging and reporting of quality control data, event records, and program information. Automatic in-process reagent rotation—no manual handling of reagents Three distinct fill levels in chamber to shorten processing times and ten customized programs and five flush options Easy-to-read screens with colorful graphics.
complimentary service with the molecular core